biostudies-literatureOufensou SConsiglio Nazionale delle RicercheConsejo Nacional de Investigaciones Cient?ficas y T?cnicasFondo per lo Sviluppo e la CoesioneN? Forschungs- und Bildungsges.m.b.H.Sipcam Italia S.p.A.Fondazione di Sardegna29407-29415https://www.ebi.ac.uk/biostudies/studies/S-EPMC7676359biostudies-literatureUnknown5(45)To assess the in vitro activity of five naturally occurring phenolic compounds (ferulic acid, apocynin, magnolol, honokiol, and thymol) on mycelial growth and type B trichothecene mycotoxin accumulation by <i>Fusarium graminearum</i>, three complementary approaches were adopted. First, a high-throughput photometric continuous reading array allowed a parallel quantification of <i>F. graminearum</i> hyphal growth and reporter <i>TRI5</i> gene expression directly on solid medium. Second, RT-qPCR confirmed the regulation of <i>TRI5</i> expression by the tested compounds. Third, liquid chromatography-tandem mass spectrometry analysis allowed quantification of deoxynivalenol (DON) and its acetylated forms released upon treatment with the phenolic compounds. Altogether, the results confirmed the ACS omegaNaturally Occurring Phenols Modulate Vegetative Growth and Deoxynivalenol Biosynthesis in <i>Fusarium graminearum</i>.PMC76763592016-2018RASSR73282K3-G-2/026-2013CUP: J86C160000700092016-20192017/2018Fabbri DDettori MAStrauss JDelogu GOufensou SZehetbauer FMigheli QBalmas VSchuller CAzara EfalseNaturally Occurring Phenols Modulate Vegetative Growth and Deoxynivalenol Biosynthesis in <i>Fusarium graminearum</i>.To assess the in vitro activity of five naturally occurring phenolic compounds (ferulic acid, apocynin, magnolol, honokiol, and thymol) on mycelial growth and type B trichothecene mycotoxin accumulation by <i>Fusarium graminearum</i>, three complementary approaches were adopted. First, a high-throughput photometric continuous reading array allowed a parallel quantification of <i>F. graminearum</i> hyphal growth and reporter <i>TRI5</i> gene expression directly on solid medium. Second, RT-qPCR confirmed the regulation of <i>TRI5</i> expression by the tested compounds. Third, liquid chromatography-tandem mass spectrometry analysis allowed quantification of deoxynivalenol (DON) and its acetylated forms released upon treatment with the phenolic compounds. Altogether, the results confirmed the 2020-01-01T00:00:00Z2020 Nov2025-04-18T21:37:52.581Z2025-04-07T09:29:41.206ZS-EPMC76763593322517210.1021/acsomega.0c04260