biostudies-literatureUnknown5(6)Jakkala KDBT-IISc Partnership ProgrammeThe emergence of antibiotic genetic resisters of pathogenic bacteria poses a major public health challenge. The mechanism by which bacterial antibiotic genetic resister clones formed <i>de novo</i> multiply and establish a resister population remained unknown. Here, we delineated the unique mode of cell division of the antibiotic genetic resisters of <i>Mycobacterium smegmatis</i> and <i>Mycobacterium tuberculosis</i> formed <i>de novo</i> from the population surviving in the presence of bactericidal concentrations of rifampicin or moxifloxacin. The cells in the rifampicin/moxifloxacin-surviving population generated elevated levels of hydroxyl radical-inflicting mutations. The genetic mutants selected against rifampicin/moxifloxacin became multinucleated and multiseptated and developed mulmSpheree00994-20https://www.ebi.ac.uk/biostudies/studies/S-EPMC7677009biostudies-literatureUnique Mode of Cell Division by the Mycobacterial Genetic Resister Clones Emerging <i>De Novo</i> from the Antibiotic-Surviving Population.PMC7677009Pradhan AAjitkumar PSharan DPaul ASwaminath SNair RRJakkala KfalseUnique Mode of Cell Division by the Mycobacterial Genetic Resister Clones Emerging <i>De Novo</i> from the Antibiotic-Surviving Population.The emergence of antibiotic genetic resisters of pathogenic bacteria poses a major public health challenge. The mechanism by which bacterial antibiotic genetic resister clones formed <i>de novo</i> multiply and establish a resister population remained unknown. Here, we delineated the unique mode of cell division of the antibiotic genetic resisters of <i>Mycobacterium smegmatis</i> and <i>Mycobacterium tuberculosis</i> formed <i>de novo</i> from the population surviving in the presence of bactericidal concentrations of rifampicin or moxifloxacin. The cells in the rifampicin/moxifloxacin-surviving population generated elevated levels of hydroxyl radical-inflicting mutations. The genetic mutants selected against rifampicin/moxifloxacin became multinucleated and multiseptated and developed mul2020-01-01T00:00:00Z2020 Nov2026-06-06T03:30:45.92Z2021-02-20T02:47:44ZS-EPMC76770093320851910.1128/mSphere.00994-20