biostudies-literatureChen JEMayo Clinic Brain Tumor Patient-Derived Xenograft National ResourceNIBIB NIH HHSNIDDK NIH HHSNational Institute of Diabetes and Digestive and Kidney DiseasesNational Cancer InstituteNCI NIH HHSNINDS NIH HHSNational Institute of Biomedical Imaging and Bioengineering346https://www.ebi.ac.uk/biostudies/studies/S-EPMC7677841biostudies-literatureUnknown17(1)<h4>Background</h4>Glioblastoma is the most common and deadly form of primary brain cancer, accounting for more than 13,000 new diagnoses annually in the USA alone. Microglia are the innate immune cells within the central nervous system, acting as a front-line defense against injuries and inflammation via a process that involves transformation from a quiescent to an activated phenotype. Crosstalk between GBM cells and microglia represents an important axis to consider in the development of tissue engineering platforms to examine pathophysiological processes underlying GBM progression and therapy.<h4>Methods</h4>This work used a brain-mimetic hydrogel system to study patient-derived glioblastoma specimens and their interactions with microglia. Here, glioblastoma cells were either cultured aJournal of neuroinflammationCrosstalk between microglia and patient-derived glioblastoma cells inhibit invasion in a three-dimensional gelatin hydrogel model.PMC7677841P50 CA108961R01 DK099528R24 NS092940R01 CA197488T32 CA009370T32 EB019944R01CA197488R01DK099528T32EB019944Gaskins HRLumibao JSteelman AJHarley BACChen JELeary SSarkaria JNfalseCrosstalk between microglia and patient-derived glioblastoma cells inhibit invasion in a three-dimensional gelatin hydrogel model.<h4>Background</h4>Glioblastoma is the most common and deadly form of primary brain cancer, accounting for more than 13,000 new diagnoses annually in the USA alone. Microglia are the innate immune cells within the central nervous system, acting as a front-line defense against injuries and inflammation via a process that involves transformation from a quiescent to an activated phenotype. Crosstalk between GBM cells and microglia represents an important axis to consider in the development of tissue engineering platforms to examine pathophysiological processes underlying GBM progression and therapy.<h4>Methods</h4>This work used a brain-mimetic hydrogel system to study patient-derived glioblastoma specimens and their interactions with microglia. Here, glioblastoma cells were either cultured a2020-01-01T00:00:00Z2020 Nov2025-04-18T14:43:10.51Z2024-11-12T23:46:40.653ZS-EPMC76778413320815610.1186/s12974-020-02026-6