biostudies-literatureFarrell KMWisconsin Alumni Research FoundationNIDDK NIH HHSNational Institutes of HealthNIGMS NIH HHSNational Science FoundationOffice of the Vice Chancellor for Research and Graduate Education, University of Wisconsin-Madison33584-33602https://www.ebi.ac.uk/biostudies/studies/S-EPMC7679191biostudies-literatureUnknown28(22)The majority of 2D IR spectrometers operate at 1-10 kHz using Ti:Sapphire laser technology. We report a 2D IR spectrometer designed around Yb:KGW laser technology that operates shot-to-shot at 100 kHz. It includes a home-built OPA, a mid-IR pulse shaper, and custom-designed electronics with optional on-chip processing. We report a direct comparison between Yb:KGW and Ti:Sapphire based 2D IR spectrometers. Even though the mid-IR pulse energy is much lower for the Yb:KGW driven system, there is an 8x improvement in signal-to-noise over the 1 kHz Ti:Sapphire driven spectrometer to which it is compared. Experimental data is shown for sub-millimolar concentrations of amides. Advantages and disadvantages of the design are discussed, including thermal background that arises at high repetition ratOptics expressShot-to-shot 2D IR spectroscopy at 100 kHz using a Yb laser and custom-designed electronics.PMC7679191R01DK079895R01 GM102387DGE-1747503R01 DK079895R01GM102387P30 DK020579Ostrander JSFarrell KMJones ACDicke SSYakami BRZanni MTMiddleton CTHamm PfalseShot-to-shot 2D IR spectroscopy at 100 kHz using a Yb laser and custom-designed electronics.The majority of 2D IR spectrometers operate at 1-10 kHz using Ti:Sapphire laser technology. We report a 2D IR spectrometer designed around Yb:KGW laser technology that operates shot-to-shot at 100 kHz. It includes a home-built OPA, a mid-IR pulse shaper, and custom-designed electronics with optional on-chip processing. We report a direct comparison between Yb:KGW and Ti:Sapphire based 2D IR spectrometers. Even though the mid-IR pulse energy is much lower for the Yb:KGW driven system, there is an 8x improvement in signal-to-noise over the 1 kHz Ti:Sapphire driven spectrometer to which it is compared. Experimental data is shown for sub-millimolar concentrations of amides. Advantages and disadvantages of the design are discussed, including thermal background that arises at high repetition rat2020-01-01T00:00:00Z2020 Oct2026-07-16T00:39:26.186Z2026-07-09T10:26:31.041ZS-EPMC76791913311501810.1364/OE.40936010.1364/oe.409360