{"database":"biostudies-literature","file_versions":[],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":54,"searchCount":0},"additional":{"submitter":["Ye Q"],"funding":["National Basic Research Program of China","National Natural Science Foundation of China"],"pagination":["544374"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7680865"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11"],"pubmed_abstract":["Temperature is considered one of the critical factors directly influencing grapevine during the three primary growth and development stages: sprout, flowering, and fruit-coloring, which is strongly correlated to the yield and quality of the grape. The grapevine is frequently exposed to high-temperature conditions that are detrimental to growth. However, the mechanisms of the heat stress response and adaptation in grapevine are not adequately studied. The <i>Arabidopsis</i> copine gene <i>AtBON1</i> encodes a highly conserved protein containing two C2 domains at the amino terminus, participation in cell death regulation and defense responses. Previously, we showed that a BON1 association protein from the grapevine, <i>VvBAP1</i>, plays a positive role in cold tolerance. Similarly, the involvement of <i>VvBAP1</i> in the resistance to heat stress was also found in the present study. The results indicated <i>VvBAP1</i> was significantly induced by high temperature, and the elevated expression of <i>VvBAP1</i> was significantly higher in the resistant cultivars than the sensitive cultivars under heat stress. Seed germination and phenotypic analysis results indicated that overexpression of <i>VvBAP1</i> improved <i>Arabidopsis</i> thermoresistance. Compared with the wild type, the chlorophyll content and net photosynthetic rate in <i>VvBAP1</i> overexpressing <i>Arabidopsis</i> plants were markedly increased under heat stress. At high temperatures, overexpression of <i>VvBAP1</i> also enhanced antioxidant enzyme activity as well as their corresponding gene transcription levels, to reduce the accumulation of reactive oxygen species and lipid peroxidation. Besides, the transcriptional activities of <i>HSP70</i>, <i>HSP101</i>, <i>HSFA2</i>, and <i>HSFB1</i> in <i>VvBAP1</i> overexpressing <i>Arabidopsis</i> plants were significantly up-regulated compare to the wild type. In summary, we propose that <i>VvBAP1</i> may play a potential important role in enhanced grapevine thermoresistance, primarily through the enhancement of antioxidant enzyme activity and promoted heat stress response genes expression."],"journal":["Frontiers in plant science"],"pubmed_title":["VvBAP1, a Grape C2 Domain Protein, Plays a Positive Regulatory Role Under Heat Stress."],"pmcid":["PMC7680865"],"funding_grant_id":["Grant No. 31572107 and 31872082","Grant No. 2018YFD1000302"],"pubmed_authors":["Hou L","Liu X","Ye Q","Zhang Z","Yu J"],"view_count":["54"],"additional_accession":[]},"is_claimable":false,"name":"VvBAP1, a Grape C2 Domain Protein, Plays a Positive Regulatory Role Under Heat Stress.","description":"Temperature is considered one of the critical factors directly influencing grapevine during the three primary growth and development stages: sprout, flowering, and fruit-coloring, which is strongly correlated to the yield and quality of the grape. The grapevine is frequently exposed to high-temperature conditions that are detrimental to growth. However, the mechanisms of the heat stress response and adaptation in grapevine are not adequately studied. The <i>Arabidopsis</i> copine gene <i>AtBON1</i> encodes a highly conserved protein containing two C2 domains at the amino terminus, participation in cell death regulation and defense responses. Previously, we showed that a BON1 association protein from the grapevine, <i>VvBAP1</i>, plays a positive role in cold tolerance. Similarly, the involvement of <i>VvBAP1</i> in the resistance to heat stress was also found in the present study. The results indicated <i>VvBAP1</i> was significantly induced by high temperature, and the elevated expression of <i>VvBAP1</i> was significantly higher in the resistant cultivars than the sensitive cultivars under heat stress. Seed germination and phenotypic analysis results indicated that overexpression of <i>VvBAP1</i> improved <i>Arabidopsis</i> thermoresistance. Compared with the wild type, the chlorophyll content and net photosynthetic rate in <i>VvBAP1</i> overexpressing <i>Arabidopsis</i> plants were markedly increased under heat stress. At high temperatures, overexpression of <i>VvBAP1</i> also enhanced antioxidant enzyme activity as well as their corresponding gene transcription levels, to reduce the accumulation of reactive oxygen species and lipid peroxidation. Besides, the transcriptional activities of <i>HSP70</i>, <i>HSP101</i>, <i>HSFA2</i>, and <i>HSFB1</i> in <i>VvBAP1</i> overexpressing <i>Arabidopsis</i> plants were significantly up-regulated compare to the wild type. In summary, we propose that <i>VvBAP1</i> may play a potential important role in enhanced grapevine thermoresistance, primarily through the enhancement of antioxidant enzyme activity and promoted heat stress response genes expression.","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020","modification":"2024-11-06T05:32:19.564Z","creation":"2021-02-20T00:25:11Z"},"accession":"S-EPMC7680865","cross_references":{"pubmed":["33240290"],"doi":["10.3389/fpls.2020.544374"]}}