{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Muller TR"],"funding":["Deutsches Zentrum für Infektionsforschung"],"pagination":["e1216"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7681835"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(11)"],"pubmed_abstract":["<h4>Objective</h4>Transgenic re-expression enables unbiased investigation of T-cell receptor (TCR)-intrinsic characteristics detached from its original cellular context. Recent advancements in TCR repertoire sequencing and development of protocols for direct cloning of full TCRαβ constructs now facilitate large-scale transgenic TCR re-expression. Together, this offers unprecedented opportunities for the screening of TCRs for basic research as well as clinical use. However, the functional characterisation of re-expressed TCRs is still a complicated and laborious matter. Here, we propose a Jurkat-based triple parameter TCR signalling reporter endogenous TCR knockout cellular platform (TPR<sup>KO</sup>) that offers an unbiased, easy read-out of TCR functionality and facilitates high-throughpu"],"journal":["Clinical & translational immunology"],"pubmed_title":["A T-cell reporter platform for high-throughput and reliable investigation of TCR function and biology."],"pmcid":["PMC7681835"],"funding_grant_id":["FKZ8023807818"],"pubmed_authors":["Kohler A","Leitner J","Muller TR","Jutz S","Schober K","Busch DH","Steinberger P","Schuler C","Hammel M"],"additional_accession":[]},"is_claimable":false,"name":"A T-cell reporter platform for high-throughput and reliable investigation of TCR function and biology.","description":"<h4>Objective</h4>Transgenic re-expression enables unbiased investigation of T-cell receptor (TCR)-intrinsic characteristics detached from its original cellular context. Recent advancements in TCR repertoire sequencing and development of protocols for direct cloning of full TCRαβ constructs now facilitate large-scale transgenic TCR re-expression. Together, this offers unprecedented opportunities for the screening of TCRs for basic research as well as clinical use. However, the functional characterisation of re-expressed TCRs is still a complicated and laborious matter. Here, we propose a Jurkat-based triple parameter TCR signalling reporter endogenous TCR knockout cellular platform (TPR<sup>KO</sup>) that offers an unbiased, easy read-out of TCR functionality and facilitates high-throughpu","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020","modification":"2025-04-19T06:53:44.325Z","creation":"2021-02-20T02:52:31Z"},"accession":"S-EPMC7681835","cross_references":{"pubmed":["33251011"],"doi":["10.1002/cti2.1216"]}}