biostudies-literatureMuller TRDeutsches Zentrum für Infektionsforschunge1216https://www.ebi.ac.uk/biostudies/studies/S-EPMC7681835biostudies-literatureUnknown9(11)<h4>Objective</h4>Transgenic re-expression enables unbiased investigation of T-cell receptor (TCR)-intrinsic characteristics detached from its original cellular context. Recent advancements in TCR repertoire sequencing and development of protocols for direct cloning of full TCRαβ constructs now facilitate large-scale transgenic TCR re-expression. Together, this offers unprecedented opportunities for the screening of TCRs for basic research as well as clinical use. However, the functional characterisation of re-expressed TCRs is still a complicated and laborious matter. Here, we propose a Jurkat-based triple parameter TCR signalling reporter endogenous TCR knockout cellular platform (TPR^KO) that offers an unbiased, easy read-out of TCR functionality and facilitates high-throughpuClinical & translational immunologyA T-cell reporter platform for high-throughput and reliable investigation of TCR function and biology.PMC7681835FKZ8023807818Kohler ALeitner JMuller TRJutz SSchober KBusch DHSteinberger PSchuler CHammel MfalseA T-cell reporter platform for high-throughput and reliable investigation of TCR function and biology.<h4>Objective</h4>Transgenic re-expression enables unbiased investigation of T-cell receptor (TCR)-intrinsic characteristics detached from its original cellular context. Recent advancements in TCR repertoire sequencing and development of protocols for direct cloning of full TCRαβ constructs now facilitate large-scale transgenic TCR re-expression. Together, this offers unprecedented opportunities for the screening of TCRs for basic research as well as clinical use. However, the functional characterisation of re-expressed TCRs is still a complicated and laborious matter. Here, we propose a Jurkat-based triple parameter TCR signalling reporter endogenous TCR knockout cellular platform (TPR^KO) that offers an unbiased, easy read-out of TCR functionality and facilitates high-throughpu2020-01-01T00:00:00Z20202025-04-19T06:53:44.325Z2021-02-20T02:52:31ZS-EPMC76818353325101110.1002/cti2.1216