{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Tang R"],"funding":["Tobacco-Related Disease Research Program","Stanford University","Stanford University School of Medicine","National Cancer Institute","National Institutes of Health","National Science Foundation"],"pagination":["e61080"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7682987"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9"],"pubmed_abstract":["Cell-cell interactions influence all aspects of development, homeostasis, and disease. In cancer, interactions between cancer cells and stromal cells play a major role in nearly every step of carcinogenesis. Thus, the ability to record cell-cell interactions would facilitate mechanistic delineation of the role of the cancer microenvironment. Here, we describe GFP-based Touching Nexus (G-baToN) which relies upon nanobody-directed fluorescent protein transfer to enable sensitive and specific labeling of cells after cell-cell interactions. G-baToN is a generalizable system that enables physical contact-based labeling between various human and mouse cell types, including endothelial cell-pericyte, neuron-astrocyte, and diverse cancer-stromal cell pairs. A suite of orthogonal baToN tools enable"],"journal":["eLife"],"pubmed_title":["A versatile system to record cell-cell interactions."],"pmcid":["PMC7682987"],"funding_grant_id":["R01-CA230919","R01-CA175336","27FT-0044","R01-CA207133","K25-CA201545","Dean’s Postdoctoral Fellowship","Anne T. and Robert M. Bass Fellowship","CA196029","Graduate Research Fellowship Program"],"pubmed_authors":["Winslow MM","Chen LC","Gitler AD","Lyu Z","Murray CW","Tsai MK","Tang R","Engleman E","Linde IL","Lee W","Kramer NJ","Cai H"],"additional_accession":[]},"is_claimable":false,"name":"A versatile system to record cell-cell interactions.","description":"Cell-cell interactions influence all aspects of development, homeostasis, and disease. In cancer, interactions between cancer cells and stromal cells play a major role in nearly every step of carcinogenesis. Thus, the ability to record cell-cell interactions would facilitate mechanistic delineation of the role of the cancer microenvironment. Here, we describe GFP-based Touching Nexus (G-baToN) which relies upon nanobody-directed fluorescent protein transfer to enable sensitive and specific labeling of cells after cell-cell interactions. G-baToN is a generalizable system that enables physical contact-based labeling between various human and mouse cell types, including endothelial cell-pericyte, neuron-astrocyte, and diverse cancer-stromal cell pairs. A suite of orthogonal baToN tools enable","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Oct","modification":"2025-04-04T10:11:15.78Z","creation":"2021-02-20T00:27:58Z"},"accession":"S-EPMC7682987","cross_references":{"pubmed":["33025906"],"doi":["10.7554/eLife.61080"]}}