biostudies-literatureSong PNational Natural Science Foundation of China6069-6082https://www.ebi.ac.uk/biostudies/studies/S-EPMC7684604biostudies-literatureUnknown8(11)<i>Fructus Rosae Roxburghii</i> (FRR) as a dietary supplement is considered to possess anti-atherosclerosis (AS), and hyperlipidemia (HLP) is material basis for AS formation, so the effects and molecular mechanism of FRR on diet-induced hyperlipidemic mice were explored. In Diet IV2 group, hepatic steatosis was significantly relieved; meanwhile, TC, TG, LDL-C, HDL-C, and ASI in serum were regulated to control level. Thirty-seven DCEG in Diet I, Diet II, and Diet IV2 groups were obtained by RNA-seq analysis. Relative mRNA levels were further determined by qRT-PCR, of which 28 genes were matched with those detected by RNA-seq. Ten DCEP were verified by targeted quantitative proteomic analysis, but expressive patterns of only six proteins were correlated with qRT-PCR data. These DCEG and DCEPFood science & nutritionJuice from <i>Fructus Rosae Roxburghii</i> normalizes blood lipids in mice with diet-induced hyperlipidemia*^†.PMC768460431760325U1812403‐4‐481760725Song PShen XfalseJuice from <i>Fructus Rosae Roxburghii</i> normalizes blood lipids in mice with diet-induced hyperlipidemia*^†.<i>Fructus Rosae Roxburghii</i> (FRR) as a dietary supplement is considered to possess anti-atherosclerosis (AS), and hyperlipidemia (HLP) is material basis for AS formation, so the effects and molecular mechanism of FRR on diet-induced hyperlipidemic mice were explored. In Diet IV2 group, hepatic steatosis was significantly relieved; meanwhile, TC, TG, LDL-C, HDL-C, and ASI in serum were regulated to control level. Thirty-seven DCEG in Diet I, Diet II, and Diet IV2 groups were obtained by RNA-seq analysis. Relative mRNA levels were further determined by qRT-PCR, of which 28 genes were matched with those detected by RNA-seq. Ten DCEP were verified by targeted quantitative proteomic analysis, but expressive patterns of only six proteins were correlated with qRT-PCR data. These DCEG and DCEP2020-01-01T00:00:00Z2020 Nov2025-04-04T22:36:44.457Z2021-02-20T10:38:19ZS-EPMC76846043328225910.1002/fsn3.1897