biostudies-literatureBueno DFPROADI-SUS501https://www.ebi.ac.uk/biostudies/studies/S-EPMC7687766biostudies-literatureUnknown11(1)<h4>Background</h4>Bone reconstruction in congenital craniofacial differences, which affect about 2-3% of newborns, has long been the focus of intensive research in the field of bone tissue engineering. The possibility of using mesenchymal stromal cells in regenerative medicine protocols has opened a new field of investigation aimed at finding optimal sources of multipotent cells that can be isolated via non-invasive procedures. In this study, we analyzed whether levator veli palatini muscle fragments, which can be readily obtained in non-invasive manner during palatoplasty in cleft palate patients, represent a novel source of MSCs with osteogenic potential.<h4>Methods</h4>We obtained levator veli palatini muscle fragments (3-5 mm³), during surgical repair of cleft palate in 5 uStem cell research & therapyHuman levator veli palatini muscle: a novel source of mesenchymal stromal cells for use in the rehabilitation of patients with congenital craniofacial malformations.PMC768776625000.009809/2018-65Rocha DLShibuya YKabayashi GSTanikawa DYSRaposo-Amaral CEZuK PAFerreira JRMPassos-Bueno MRHokugo AJarrahy RBueno DFPinheiro CCGfalseHuman levator veli palatini muscle: a novel source of mesenchymal stromal cells for use in the rehabilitation of patients with congenital craniofacial malformations.<h4>Background</h4>Bone reconstruction in congenital craniofacial differences, which affect about 2-3% of newborns, has long been the focus of intensive research in the field of bone tissue engineering. The possibility of using mesenchymal stromal cells in regenerative medicine protocols has opened a new field of investigation aimed at finding optimal sources of multipotent cells that can be isolated via non-invasive procedures. In this study, we analyzed whether levator veli palatini muscle fragments, which can be readily obtained in non-invasive manner during palatoplasty in cleft palate patients, represent a novel source of MSCs with osteogenic potential.<h4>Methods</h4>We obtained levator veli palatini muscle fragments (3-5 mm³), during surgical repair of cleft palate in 5 u2020-01-01T00:00:00Z2020 Nov2026-04-30T06:23:03.217Z2021-02-20T03:03:52ZS-EPMC76877663323908010.1186/s13287-020-02017-7