biostudies-literatureDobreva TNINDS NIH HHS20540https://www.ebi.ac.uk/biostudies/studies/S-EPMC7688970biostudies-literatureUnknown10(1)An individual's immune system is driven by both genetic and environmental factors that vary over time. To better understand the temporal and inter-individual variability of gene expression within distinct immune cell types, we developed a platform that leverages multiplexed single-cell sequencing and out-of-clinic capillary blood extraction to enable simplified, cost-effective profiling of the human immune system across people and time at single-cell resolution. Using the platform, we detect widespread differences in cell type-specific gene expression between subjects that are stable over multiple days.Scientific reportsSingle cell profiling of capillary blood enables out of clinic human immunity studies.PMC7688970T32 NS105595Park JHThomson MDobreva TBrown DfalseSingle cell profiling of capillary blood enables out of clinic human immunity studies.An individual's immune system is driven by both genetic and environmental factors that vary over time. To better understand the temporal and inter-individual variability of gene expression within distinct immune cell types, we developed a platform that leverages multiplexed single-cell sequencing and out-of-clinic capillary blood extraction to enable simplified, cost-effective profiling of the human immune system across people and time at single-cell resolution. Using the platform, we detect widespread differences in cell type-specific gene expression between subjects that are stable over multiple days.2020-01-01T00:00:00Z2020 Nov2025-04-27T02:55:50.035Z2021-02-21T04:54:08ZS-EPMC76889703323969010.1038/s41598-020-77073-3