biostudies-literatureAmelkina OSmithsonian InstitutionNIH HHS828https://www.ebi.ac.uk/biostudies/studies/S-EPMC7690003biostudies-literatureUnknown21(1)<h4>Background</h4>Long term preservation of living ovarian tissues is a critical approach in human reproductive medicine as well as in the conservation of rare animal genotypes. Compared to single cell preservation, optimization of protocols for tissues is highly complex because of the diversity of cells responding differently to non-physiological conditions. Using the prepubertal domestic cat as a model, the objective was to study immediate effects of vitrification or microwave-assisted dehydration on the global transcriptome dynamics in the ovarian cortex. RNA sequencing was performed on ovarian tissues (n = 6 individuals) from different conditions: fresh tissue after dissection (F), vitrified/warmed tissue (V), tissue dehydrated for 5 min (D5) or 10 min (D10) followed by rehydration. DBMC genomicsInitial response of ovarian tissue transcriptome to vitrification or microwave-assisted dehydration in the domestic cat model.PMC7690003R01 OD023139Amelkina OComizzoli PfalseInitial response of ovarian tissue transcriptome to vitrification or microwave-assisted dehydration in the domestic cat model.<h4>Background</h4>Long term preservation of living ovarian tissues is a critical approach in human reproductive medicine as well as in the conservation of rare animal genotypes. Compared to single cell preservation, optimization of protocols for tissues is highly complex because of the diversity of cells responding differently to non-physiological conditions. Using the prepubertal domestic cat as a model, the objective was to study immediate effects of vitrification or microwave-assisted dehydration on the global transcriptome dynamics in the ovarian cortex. RNA sequencing was performed on ovarian tissues (n = 6 individuals) from different conditions: fresh tissue after dissection (F), vitrified/warmed tissue (V), tissue dehydrated for 5 min (D5) or 10 min (D10) followed by rehydration. D2020-01-01T00:00:00Z2020 Nov2026-05-28T11:26:57.874Z2025-02-19T03:26:24.177ZS-EPMC76900033323887810.1186/s12864-020-07236-z