{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Shilts MH"],"funding":["National Institute of Allergy and Infectious Diseases","Eunice Kennedy Shriver National Institute of Child Health and Human Development","NCATS NIH HHS","NEI NIH HHS","NIAID, NIH","NIDDK NIH HHS","NCRR NIH HHS","National Institute of Diabetes and Digestive and Kidney Diseases","NIAID NIH HHS","Parker B. Francis Fellowship Program","NCI NIH HHS","NIDDK"],"pagination":["20618"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7692476"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(1)"],"pubmed_abstract":["Despite being commonly used to collect upper airway epithelial lining fluid, nasal washes are poorly reproducible, not suitable for serial sampling, and limited by a dilution effect. In contrast, nasal filters lack these limitations and are an attractive alternative. To examine whether nasal filters are superior to nasal washes as a sampling method for the characterization of the upper airway microbiome and immune response, we collected paired nasal filters and washes from a group of 40 healthy children and adults. To characterize the upper airway microbiome, we used 16S ribosomal RNA and shotgun metagenomic sequencing. To characterize the immune response, we measured total protein using a BCA assay and 53 immune mediators using multiplex magnetic bead-based assays. We conducted statistica"],"journal":["Scientific reports"],"pubmed_title":["Evaluation of the upper airway microbiome and immune response with nasal epithelial lining fluid absorption and nasal washes."],"pmcid":["PMC7692476"],"funding_grant_id":["U19AI095227","P30DK020593","K24AI77930","K12HD087023","UL1 TR000445","P30 EY008126","U2CDK059637","UL1 RR024975","P30 CA068485","G20 RR030956","HHSN272200900007C","R21 AI149262","R21 AI154016","U19 AI110819"],"pubmed_authors":["Boone HH","Peebles RS","Russell PB","Wiggins DA","Tovchigrechko A","Costello KM","Hartert TV","McCollum MD","Rosas-Salazar C","Yooseph S","Connolly AS","Rajagopala SV","Mai A","Das SR","Shilts MH","Lynch CE"],"additional_accession":[]},"is_claimable":false,"name":"Evaluation of the upper airway microbiome and immune response with nasal epithelial lining fluid absorption and nasal washes.","description":"Despite being commonly used to collect upper airway epithelial lining fluid, nasal washes are poorly reproducible, not suitable for serial sampling, and limited by a dilution effect. In contrast, nasal filters lack these limitations and are an attractive alternative. To examine whether nasal filters are superior to nasal washes as a sampling method for the characterization of the upper airway microbiome and immune response, we collected paired nasal filters and washes from a group of 40 healthy children and adults. To characterize the upper airway microbiome, we used 16S ribosomal RNA and shotgun metagenomic sequencing. To characterize the immune response, we measured total protein using a BCA assay and 53 immune mediators using multiplex magnetic bead-based assays. We conducted statistica","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Nov","modification":"2025-04-22T18:26:43.612Z","creation":"2025-04-06T02:25:03.97Z"},"accession":"S-EPMC7692476","cross_references":{"pubmed":["33244064"],"doi":["10.1038/s41598-020-77289-3"]}}