{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Groves NS"],"funding":["NIAID NIH HHS","Division of Intramural Research, National Institute of Allergy and Infectious Diseases"],"pagination":["E972"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7700196"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(11)"],"pubmed_abstract":["The insurgence of superresolution microscopy into the fields of virology and microbiology has begun to enable the mapping of molecular assemblies critical for host-pathogen interfaces that organize on a scale below the resolution limit of the light microscope. It is, however, challenging to completely understand the molecular interactions between host and pathogen from strictly time-invariant observations. Herein, we describe a method using simultaneous dual-color superresolution microscopy to gain both structural and dynamic information about HIV-1 assembly. Specifically, we demonstrate the reconstruction of single virus assembly sites using live-cell photo-activated localization microscopy (PALM) while concurrently assessing the sub-viral mobility of the HIV-1 envelope glycoprotein during interaction with the viral lattice. We propose that our method is broadly applicable to elucidating pathogen and host protein-protein interactions through quantification of the dynamics of these proteins at the nanoscale."],"journal":["Pathogens (Basel, Switzerland)"],"pubmed_title":["A Quantitative Live-Cell Superresolution Imaging Framework for Measuring the Mobility of Single Molecules at Sites of Virus Assembly."],"pmcid":["PMC7700196"],"funding_grant_id":["R01 AI138625","R01AI138625"],"pubmed_authors":["Groves NS","Bruns MM","van Engelenburg SB"],"additional_accession":[]},"is_claimable":false,"name":"A Quantitative Live-Cell Superresolution Imaging Framework for Measuring the Mobility of Single Molecules at Sites of Virus Assembly.","description":"The insurgence of superresolution microscopy into the fields of virology and microbiology has begun to enable the mapping of molecular assemblies critical for host-pathogen interfaces that organize on a scale below the resolution limit of the light microscope. It is, however, challenging to completely understand the molecular interactions between host and pathogen from strictly time-invariant observations. Herein, we describe a method using simultaneous dual-color superresolution microscopy to gain both structural and dynamic information about HIV-1 assembly. Specifically, we demonstrate the reconstruction of single virus assembly sites using live-cell photo-activated localization microscopy (PALM) while concurrently assessing the sub-viral mobility of the HIV-1 envelope glycoprotein during interaction with the viral lattice. We propose that our method is broadly applicable to elucidating pathogen and host protein-protein interactions through quantification of the dynamics of these proteins at the nanoscale.","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Nov","modification":"2026-05-02T22:52:56.282Z","creation":"2021-02-20T03:05:49Z"},"accession":"S-EPMC7700196","cross_references":{"pubmed":["33233482"],"doi":["10.3390/pathogens9110972"]}}