{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Raote I"],"funding":["Generalitat de Catalunya","ICREA","European Research Council","Fundacio Privada Cellex","Spanish Ministry of Science and Innovation","BIST","State Research Agency","Government of Spain","María de Maeztu Unit of Excellence","Ministerio de Economía y Competitividad","Fundacio Privada Mir-Puig","Spanish Government"],"pagination":["e59426"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7704110"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9"],"pubmed_abstract":["The endoplasmic reticulum (ER)-resident protein TANGO1 assembles into a ring around ER exit sites (ERES), and links procollagens in the ER lumen to COPII machinery, tethers, and ER-Golgi intermediate compartment (ERGIC) in the cytoplasm (Raote et al., 2018). Here, we present a theoretical approach to investigate the physical mechanisms of TANGO1 ring assembly and how COPII polymerization, membrane tension, and force facilitate the formation of a transport intermediate for procollagen export. Our results indicate that a TANGO1 ring, by acting as a linactant, stabilizes the open neck of a nascent COPII bud. Elongation of such a bud into a transport intermediate commensurate with bulky procollagens is then facilitated by two complementary mechanisms: (i) by relieving membrane tension, possibly by TANGO1-mediated fusion of retrograde ERGIC membranes and (ii) by force application. Altogether, our theoretical approach identifies key biophysical events in TANGO1-driven procollagen export."],"journal":["eLife"],"pubmed_title":["A physical mechanism of TANGO1-mediated bulky cargo export."],"pmcid":["PMC7704110"],"funding_grant_id":["ERC Advanced Grant (GA 788546)","Ignite grant eTANGO","BFU2013-44188-P","Consolider CSD2009-00016","Severo Ochoa Program CEX2018-000797- S","654148","LaserLab 4 Europe GA 654148","ICREA academia","788546","CERCA program","FIS2015-63550-R","IJCI-2017-34751","2017-SGR-1278","BFU2015-73288-JIN","FIS2017-89560-R","Severo Ochoa Program SEV-2012–0208","MDM-2015–0502","681434","RYC-2017-22227","PID2019-106232RB-I00/ 10.13039/501100011033","CoG-681434",""Severo Ochoa" Programme (CEX2019-000910-S)","Severo Ochoa Program SEV-2012-0208","Maria de Maeztu MDM-2015-0502"],"pubmed_authors":["Walani N","Arroyo M","Garcia-Parajo MF","Malhotra V","Raote I","Chabanon M","Campelo F"],"additional_accession":[]},"is_claimable":false,"name":"A physical mechanism of TANGO1-mediated bulky cargo export.","description":"The endoplasmic reticulum (ER)-resident protein TANGO1 assembles into a ring around ER exit sites (ERES), and links procollagens in the ER lumen to COPII machinery, tethers, and ER-Golgi intermediate compartment (ERGIC) in the cytoplasm (Raote et al., 2018). Here, we present a theoretical approach to investigate the physical mechanisms of TANGO1 ring assembly and how COPII polymerization, membrane tension, and force facilitate the formation of a transport intermediate for procollagen export. Our results indicate that a TANGO1 ring, by acting as a linactant, stabilizes the open neck of a nascent COPII bud. Elongation of such a bud into a transport intermediate commensurate with bulky procollagens is then facilitated by two complementary mechanisms: (i) by relieving membrane tension, possibly by TANGO1-mediated fusion of retrograde ERGIC membranes and (ii) by force application. Altogether, our theoretical approach identifies key biophysical events in TANGO1-driven procollagen export.","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Nov","modification":"2025-04-05T12:54:41.19Z","creation":"2025-04-05T12:54:41.19Z"},"accession":"S-EPMC7704110","cross_references":{"pubmed":["33169667"],"doi":["10.7554/eLife.59426"]}}