biostudies-literatureUnknown21(1)McDonald RCAndrews University<h4>Background</h4>Like most major enzyme families, the M14 family of metallocarboxypeptidases (MCPs) contains a number of pseudoenzymes predicted to lack enzyme activity and with poorly characterized molecular function. The genome of the yeast Saccharomyces cerevisiae encodes one member of the M14 MCP family, a pseudoenzyme named Ecm14 proposed to function in the extracellular matrix. In order to better understand the function of such pseudoenzymes, we studied the structure and function of Ecm14 in S. cerevisiae.<h4>Results</h4>A phylogenetic analysis of Ecm14 in fungi found it to be conserved throughout the ascomycete phylum, with a group of related pseudoenzymes found in basidiomycetes. To investigate the structure and function of this conserved protein, His6-tagged Ecm14 was overexpresBMC molecular and cell biology86https://www.ebi.ac.uk/biostudies/studies/S-EPMC7706225biostudies-literatureBiochemical and genetic analysis of Ecm14, a conserved fungal pseudopeptidase.PMC7706225McDonald RCSchott MJIdowu TALyons PJfalseBiochemical and genetic analysis of Ecm14, a conserved fungal pseudopeptidase.<h4>Background</h4>Like most major enzyme families, the M14 family of metallocarboxypeptidases (MCPs) contains a number of pseudoenzymes predicted to lack enzyme activity and with poorly characterized molecular function. The genome of the yeast Saccharomyces cerevisiae encodes one member of the M14 MCP family, a pseudoenzyme named Ecm14 proposed to function in the extracellular matrix. In order to better understand the function of such pseudoenzymes, we studied the structure and function of Ecm14 in S. cerevisiae.<h4>Results</h4>A phylogenetic analysis of Ecm14 in fungi found it to be conserved throughout the ascomycete phylum, with a group of related pseudoenzymes found in basidiomycetes. To investigate the structure and function of this conserved protein, His6-tagged Ecm14 was overexpres2020-01-01T00:00:00Z2020 Nov2025-04-19T00:45:03.986Z2021-02-20T02:55:06ZS-EPMC77062253325660810.1186/s12860-020-00330-w