biostudies-literatureHisey EAUniversity of California Davis Provost Undergraduate Research FellowshipUniversity of California DavisUniversity of California Davis Center for Equine HealthUniversity of Califorina Davis Center for Equine HealthNational Institutes of HealthNational Institute of Food and AgricultureMorris Animal FoundationNIH HHS848https://www.ebi.ac.uk/biostudies/studies/S-EPMC7706231biostudies-literatureUnknown21(1)<h4>Background</h4>Distichiasis, an ocular disorder in which aberrant cilia (eyelashes) grow from the opening of the Meibomian glands of the eyelid, has been reported in Friesian horses. These misplaced cilia can cause discomfort, chronic keratitis, and corneal ulceration, potentially impacting vision due to corneal fibrosis, or, if secondary infection occurs, may lead to loss of the eye. Friesian horses represent the vast majority of reported cases of equine distichiasis, and as the breed is known to be affected with inherited monogenic disorders, this condition was hypothesized to be a simply inherited Mendelian trait.<h4>Results</h4>A genome wide association study (GWAS) was performed using the Axiom 670 k Equine Genotyping array (MNEc670k) utilizing 14 cases and 38 controls phenotyped BMC genomicsWhole genome sequencing identified a 16 kilobase deletion on ECA13 associated with distichiasis in Friesian horses.PMC770623117-24RT32 OD010993N/A16-125T320D010993-122017-67015-26296D16EQ-820Hisey EAHermans HKnickelbein KEAvila FLassaline MEDuward-Akhurst SALounsberry ZTGrahn RABellone RRKalbfleisch TSBack WMcCue MEfalseWhole genome sequencing identified a 16 kilobase deletion on ECA13 associated with distichiasis in Friesian horses.<h4>Background</h4>Distichiasis, an ocular disorder in which aberrant cilia (eyelashes) grow from the opening of the Meibomian glands of the eyelid, has been reported in Friesian horses. These misplaced cilia can cause discomfort, chronic keratitis, and corneal ulceration, potentially impacting vision due to corneal fibrosis, or, if secondary infection occurs, may lead to loss of the eye. Friesian horses represent the vast majority of reported cases of equine distichiasis, and as the breed is known to be affected with inherited monogenic disorders, this condition was hypothesized to be a simply inherited Mendelian trait.<h4>Results</h4>A genome wide association study (GWAS) was performed using the Axiom 670 k Equine Genotyping array (MNEc670k) utilizing 14 cases and 38 controls phenotyped 2020-01-01T00:00:00Z2020 Nov2026-05-03T05:00:25.78Z2021-02-20T03:04:45ZS-EPMC77062313325661010.1186/s12864-020-07265-8