biostudies-literatureWang XNational Natural Science Foundation of China9196379https://www.ebi.ac.uk/biostudies/studies/S-EPMC7707998biostudies-literatureUnknown2020<h4>Background</h4>Untreated nephropathy can progress to renal failure. The traditional Mongolian remedy Narenmandula regulates the kidney "yang." This study aimed to identify key microRNAs (miRNAs) targeted by Narenmandula in a rat model of nephropathy.<h4>Methods</h4>Fifteen rats exhibiting normal renal function were randomized to three study arms. Nephropathy was induced in <i>n</i> = 10 rats using doxorubicin hydrochloride, followed by either Narenmandula treatment (treatment group) or no treatment (control group). In <i>n</i> = 5 rats, no doxorubicin was given and renal function remained unchanged (healthy group). Microarray analysis identified miRNAs which were differentially expressed (DE-miRNAs) between groups. Target genes of DE-miRNAs were predicted using miRWalk version 2.0, folEvidence-based complementary and alternative medicine : eCAMIdentifying Key MicroRNAs Targeted by Narenmandula in a Rodent Nephropathy Model.PMC770799881641149Aodaofu ALiu XJin WArun ASudunabuqi SWang XChang CChen HWu FfalseIdentifying Key MicroRNAs Targeted by Narenmandula in a Rodent Nephropathy Model.<h4>Background</h4>Untreated nephropathy can progress to renal failure. The traditional Mongolian remedy Narenmandula regulates the kidney "yang." This study aimed to identify key microRNAs (miRNAs) targeted by Narenmandula in a rat model of nephropathy.<h4>Methods</h4>Fifteen rats exhibiting normal renal function were randomized to three study arms. Nephropathy was induced in <i>n</i> = 10 rats using doxorubicin hydrochloride, followed by either Narenmandula treatment (treatment group) or no treatment (control group). In <i>n</i> = 5 rats, no doxorubicin was given and renal function remained unchanged (healthy group). Microarray analysis identified miRNAs which were differentially expressed (DE-miRNAs) between groups. Target genes of DE-miRNAs were predicted using miRWalk version 2.0, fol2020-01-01T00:00:00Z20202025-04-18T11:33:01.991Z2021-02-20T10:35:32ZS-EPMC77079983329946410.1155/2020/9196379