biostudies-literatureKamps DNatural Sciences and Engineering Research Council of CanadaBundesministerium für Bildung und ForschungLeverhulme TrustDeutsche ForschungsgemeinschaftEuropean Research CouncilWolfson FoundationHorizon 2020 Framework ProgrammeIsaac Newton Institute for Mathematical SciencesStiftung MercatorKnut och Alice Wallenbergs StiftelseEngineering and Physical Sciences Research Council108467https://www.ebi.ac.uk/biostudies/studies/S-EPMC7710677biostudies-literatureUnknown33(9)Local cell contraction pulses play important roles in tissue and cell morphogenesis. Here, we improve a chemo-optogenetic approach and apply it to investigate the signal network that generates these pulses. We use these measurements to derive and parameterize a system of ordinary differential equations describing temporal signal network dynamics. Bifurcation analysis and numerical simulations predict a strong dependence of oscillatory system dynamics on the concentration of GEF-H1, an Lbc-type RhoGEF, which mediates the positive feedback amplification of Rho activity. This prediction is confirmed experimentally via optogenetic tuning of the effective GEF-H1 concentration in individual living cells. Numerical simulations show that pulse amplitude is most sensitive to external inputs into the myosin component at low GEF-H1 concentrations and that the spatial pulse width is dependent on GEF-H1 diffusion. Our study offers a theoretical framework to explain the emergence of local cell contraction pulses and their modulation by biochemical and mechanical signals.Cell reportsOptogenetic Tuning Reveals Rho Amplification-Dependent Dynamics of a Cell Contraction Signal Network.PMC7710677EP/T00410X/1EP/J016780/1Banerjee SMadzvamuse AGraessl MMazel TPortet SKamps DKoch JJuma VOCampillo-Funollet EChen XNalbant PDehmelt LWu YWfalseOptogenetic Tuning Reveals Rho Amplification-Dependent Dynamics of a Cell Contraction Signal Network.Local cell contraction pulses play important roles in tissue and cell morphogenesis. Here, we improve a chemo-optogenetic approach and apply it to investigate the signal network that generates these pulses. We use these measurements to derive and parameterize a system of ordinary differential equations describing temporal signal network dynamics. Bifurcation analysis and numerical simulations predict a strong dependence of oscillatory system dynamics on the concentration of GEF-H1, an Lbc-type RhoGEF, which mediates the positive feedback amplification of Rho activity. This prediction is confirmed experimentally via optogenetic tuning of the effective GEF-H1 concentration in individual living cells. Numerical simulations show that pulse amplitude is most sensitive to external inputs into the myosin component at low GEF-H1 concentrations and that the spatial pulse width is dependent on GEF-H1 diffusion. Our study offers a theoretical framework to explain the emergence of local cell contraction pulses and their modulation by biochemical and mechanical signals.2020-01-01T00:00:00Z2020 Dec2024-11-20T11:36:42.866Z2021-02-20T09:59:00ZS-EPMC77106773326462910.1016/j.celrep.2020.108467