{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["8(4)"],"submitter":["Michiels TJM"],"pubmed_abstract":["Currently, batch release of toxoid vaccines, such as diphtheria and tetanus toxoid, requires animal tests to confirm safety and immunogenicity. Efforts are being made to replace these tests with in vitro assays in a consistency approach. Limitations of current in vitro assays include the need for reference antigens and most are only applicable to drug substance, not to the aluminum adjuvant-containing and often multivalent drug product. To overcome these issues, a new assay was developed based on mimicking the proteolytic degradation processes in antigen-presenting cells with recombinant cathepsin S, followed by absolute quantification of the formed peptides by liquid chromatography-mass spectrometry. Temperature-exposed tetanus toxoids from several manufacturers were used as aberrant samp"],"journal":["Vaccines"],"pagination":["E712"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7712181"],"repository":["biostudies-literature"],"pubmed_title":["Degradomics-Based Analysis of Tetanus Toxoids as a Quality Control Assay."],"pmcid":["PMC7712181"],"pubmed_authors":["Kersten GFA","de Ridder JW","Jiskoot W","Danial M","Tilstra W","Meiring HD","Hamzink MRJ","Metz B","Michiels TJM"],"additional_accession":[]},"is_claimable":false,"name":"Degradomics-Based Analysis of Tetanus Toxoids as a Quality Control Assay.","description":"Currently, batch release of toxoid vaccines, such as diphtheria and tetanus toxoid, requires animal tests to confirm safety and immunogenicity. Efforts are being made to replace these tests with in vitro assays in a consistency approach. Limitations of current in vitro assays include the need for reference antigens and most are only applicable to drug substance, not to the aluminum adjuvant-containing and often multivalent drug product. To overcome these issues, a new assay was developed based on mimicking the proteolytic degradation processes in antigen-presenting cells with recombinant cathepsin S, followed by absolute quantification of the formed peptides by liquid chromatography-mass spectrometry. Temperature-exposed tetanus toxoids from several manufacturers were used as aberrant samp","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Dec","modification":"2025-04-05T17:28:52.581Z","creation":"2021-02-20T10:07:19Z"},"accession":"S-EPMC7712181","cross_references":{"pubmed":["33271767"],"doi":["10.3390/vaccines8040712"]}}