{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wafai R"],"funding":["National Health and Medical Research Council","National Breast Cancer Foundation"],"pagination":["136"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7716465"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["22(1)"],"pubmed_abstract":["Background
Breast cancers acquire aggressive capabilities via epithelial to mesenchymal transition (EMT), in which various integrins/integrin-linked kinase signalling are upregulated.Methods
We investigated this in two patient-derived xenografts (PDXs) developed from breast-to-bone metastases, and its functional significance in a breast cancer cell line system. ED03 and EDW01 PDXs were grown subcutaneously in immunocompromised SCID mice through 11 passages and 7 passages, respectively. Tumour tissue was assessed using immunohistochemistry (IHC) for oestrogen receptor (ER)-alpha, E-cadherin, vimentin, Twist1, beta-catenin, P120-RasGAP, CD44, CD24 and Ki67, and RT-qPCR of EMT-related factors (CDH1, VIM, CD44, CD24), integrins beta 1 (ITGB1), alpha 2 (ITGA2) and ILK. Integrin "],"journal":["Breast cancer research : BCR"],"pubmed_title":["Integrin alpha-2 and beta-1 expression increases through multiple generations of the EDW01 patient-derived xenograft model of breast cancer-insight into their role in epithelial mesenchymal transition in vivo gained from an in vitro model system."],"pmcid":["PMC7716465"],"funding_grant_id":["1027527","CG-10-04"],"pubmed_authors":["Williams ED","McCart Reed AE","Waltham M","Snell CE","Blick T","de Souza E","Wafai R","Kutasovic JR","Hugo HJ","Simpson PT","Thompson EW"],"additional_accession":[]},"is_claimable":false,"name":"Integrin alpha-2 and beta-1 expression increases through multiple generations of the EDW01 patient-derived xenograft model of breast cancer-insight into their role in epithelial mesenchymal transition in vivo gained from an in vitro model system.","description":"Background
Breast cancers acquire aggressive capabilities via epithelial to mesenchymal transition (EMT), in which various integrins/integrin-linked kinase signalling are upregulated.Methods
We investigated this in two patient-derived xenografts (PDXs) developed from breast-to-bone metastases, and its functional significance in a breast cancer cell line system. ED03 and EDW01 PDXs were grown subcutaneously in immunocompromised SCID mice through 11 passages and 7 passages, respectively. Tumour tissue was assessed using immunohistochemistry (IHC) for oestrogen receptor (ER)-alpha, E-cadherin, vimentin, Twist1, beta-catenin, P120-RasGAP, CD44, CD24 and Ki67, and RT-qPCR of EMT-related factors (CDH1, VIM, CD44, CD24), integrins beta 1 (ITGB1), alpha 2 (ITGA2) and ILK. Integrin ","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Dec","modification":"2025-04-22T16:52:12.785Z","creation":"2021-02-20T10:06:59Z"},"accession":"S-EPMC7716465","cross_references":{"pubmed":["33276802"],"doi":["10.1186/s13058-020-01366-8"]}}