biostudies-literatureUnknown35(12)Levin AInga-Britt and Arne Lundberg Research FoundationStrategic Research Program in Diabetes at Karolinska Institutet, Center for Innovative MedicineSahlgrenska University Hospital ALF GrantSwedish Kidney FoundationKarolinska InstitutetMargaretha af Ugglas FoundationAstraZenecaSwedish Research CouncilStockholm County Council<h4>Background</h4>Diabetic nephropathy (DN) is the most common cause of end-stage renal disease, affecting ∼30% of the rapidly growing diabetic population, and strongly associated with cardiovascular risk. Despite this, the molecular mechanisms of disease remain unknown.<h4>Methods</h4>RNA sequencing (RNAseq) was performed on paired, micro-dissected glomerular and tubulointerstitial tissue from patients diagnosed with DN [n = 19, 15 males, median (range) age: 61 (30-85) years, chronic kidney disease stages 1-4] and living kidney donors [n = 20, 12 males, median (range) age: 56 (30-70) years].<h4>Results</h4>Principal component analysis showed a clear separation between glomeruli and tubulointerstitium transcriptomes. Differential expression analysis identified 1550 and 4530 differentiallyNephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association2059-2072https://www.ebi.ac.uk/biostudies/studies/S-EPMC7716805biostudies-literatureNovel insights into the disease transcriptome of human diabetic glomeruli and tubulointerstitium.PMC7716805Levin ABlondal TErlandsson FEbefors KLiu PNordstrom JZambrano SStenvinkel PWitasp ANystrom JWernerson AReznichenko AGreasley PJBarany PPatrakka JSorrentino ABruchfeld AfalseNovel insights into the disease transcriptome of human diabetic glomeruli and tubulointerstitium.<h4>Background</h4>Diabetic nephropathy (DN) is the most common cause of end-stage renal disease, affecting ∼30% of the rapidly growing diabetic population, and strongly associated with cardiovascular risk. Despite this, the molecular mechanisms of disease remain unknown.<h4>Methods</h4>RNA sequencing (RNAseq) was performed on paired, micro-dissected glomerular and tubulointerstitial tissue from patients diagnosed with DN [n = 19, 15 males, median (range) age: 61 (30-85) years, chronic kidney disease stages 1-4] and living kidney donors [n = 20, 12 males, median (range) age: 56 (30-70) years].<h4>Results</h4>Principal component analysis showed a clear separation between glomeruli and tubulointerstitium transcriptomes. Differential expression analysis identified 1550 and 4530 differentially2020-01-01T00:00:00Z2020 Dec2025-06-01T01:55:01.549Z2025-06-01T01:55:01.549ZS-EPMC77168053285335110.1093/ndt/gfaa121