biostudies-literatureSchwitalla JWVillum FondenDeutsche Forschungsgemeinschaft5806-5811https://www.ebi.ac.uk/biostudies/studies/S-EPMC7723250biostudies-literatureUnknown70(11)The taxonomic position of a novel aerobic, Gram-positive actinobacteria, designated strain RB5^T, was determined using a polyphasic approach. The strain, isolated from the gut of the fungus-farming termite <i>Macrotermes natalensis</i>, showed morphological, physiological and chemotaxonomic properties typical of the genus <i>Streptomyces</i>. Based on 16S rRNA gene sequence analysis, the closest phylogenetic neighbour of RB5^T was <i>Streptomyces polyrhachis</i> DSM 42102^T (98.87 %). DNA-DNA hybridization experiments between strain RB5^T and <i>S. polyrhachis</i> DSM 42102^T resulted in a value of 27.4 % (26.8 %). The cell wall of strain RB5^T contained ll-diaminopimelic acid as the diagnostic amino acid. Mycolic acids and diagnostic sugars in whole-cell hydrolysates were not detected. The strain produced the following major phospholipids: diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol-mannoside and phosphatidylserine. The menaquinone profile showed hexa- and octahydrogenated menaquinones containing nine isoprene units [MK-9(H₆) and MK-9(H₈)]. The strain exhibited a fatty acid profile containing the following major fatty acids: 12-methyltridecanoic acid (iso-C_14 : 0) 12-methyltetradecanoic acid (anteiso-C_15 : 0), 13-methyltetradecanoic acid (iso-C_15 : 0) and 14-methylpentadecanoic acid (iso-C_16 : 0). Here, we propose a novel species of the genus <i>Streptomyces - Streptomyces smaragdinus</i> with the type strain RB5^T (=VKM Ac-2839^T=NRRL B65539^T).International journal of systematic and evolutionary microbiology<i>Streptomyces smaragdinus</i> sp. nov., isolated from the gut of the fungus growing-termite <i>Macrotermes natalensis</i>.PMC7723250CRC 1127 (A6) and BE-4799/3-1VKR10101Vollmers Jde Beer ZWMartin KKaster AKBenndorf RSchwitalla JWBeemelmanns CPoulsen Mfalse<i>Streptomyces smaragdinus</i> sp. nov., isolated from the gut of the fungus growing-termite <i>Macrotermes natalensis</i>.The taxonomic position of a novel aerobic, Gram-positive actinobacteria, designated strain RB5^T, was determined using a polyphasic approach. The strain, isolated from the gut of the fungus-farming termite <i>Macrotermes natalensis</i>, showed morphological, physiological and chemotaxonomic properties typical of the genus <i>Streptomyces</i>. Based on 16S rRNA gene sequence analysis, the closest phylogenetic neighbour of RB5^T was <i>Streptomyces polyrhachis</i> DSM 42102^T (98.87 %). DNA-DNA hybridization experiments between strain RB5^T and <i>S. polyrhachis</i> DSM 42102^T resulted in a value of 27.4 % (26.8 %). The cell wall of strain RB5^T contained ll-diaminopimelic acid as the diagnostic amino acid. Mycolic acids and diagnostic sugars in whole-cell hydrolysates were not detected. The strain produced the following major phospholipids: diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol-mannoside and phosphatidylserine. The menaquinone profile showed hexa- and octahydrogenated menaquinones containing nine isoprene units [MK-9(H₆) and MK-9(H₈)]. The strain exhibited a fatty acid profile containing the following major fatty acids: 12-methyltridecanoic acid (iso-C_14 : 0) 12-methyltetradecanoic acid (anteiso-C_15 : 0), 13-methyltetradecanoic acid (iso-C_15 : 0) and 14-methylpentadecanoic acid (iso-C_16 : 0). Here, we propose a novel species of the genus <i>Streptomyces - Streptomyces smaragdinus</i> with the type strain RB5^T (=VKM Ac-2839^T=NRRL B65539^T).2020-01-01T00:00:00Z2020 Nov2024-11-20T11:36:36.008Z2021-02-20T09:59:00ZS-EPMC77232503296978510.1099/ijsem.0.004478