{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(11)"],"submitter":["Zhang Y"],"pubmed_abstract":["<h4>Background</h4>Amyotrophic lateral sclerosis (ALS) can result in the dysfunction of upper and lower motor neurons. A previous study has indicated that TBK1 mutation (hTBK1-c.978T>A) is involved in progression of ALS. However, the mechanism by which TBK1 mutation mediates the progression of ALS remains unclear.<h4>Methods</h4>NSC-34 cells with hTBK1-c.978T>A mutation (TBK1 mutation status) was used to mimic ALS <i>in vitro</i>. In addition, cell proliferation was detected by Ki67 staining. Gene and protein expressions in NSC-34 cells were detected by RT-qPCR and western blot, respectively. ROS and PGSK levels in NSC-34 cells were detected by flow cytometry.<h4>Results</h4>hTBK1-c.978T>A mutation significantly inhibited the proliferation of NSC-34 cells via inducing cell ferroptosis, whi"],"journal":["American journal of translational research"],"pagination":["7386-7394"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7724361"],"repository":["biostudies-literature"],"pubmed_title":["hTBK1-c.978T>A mutation promotes the ferroptosis in NSC-34 cells via mediation of KEAP1/NRF2/p62 signaling."],"pmcid":["PMC7724361"],"pubmed_authors":["Liu X","Tang L","He J","Zhang Y","Zhang N","Fan D"],"additional_accession":[]},"is_claimable":false,"name":"hTBK1-c.978T>A mutation promotes the ferroptosis in NSC-34 cells via mediation of KEAP1/NRF2/p62 signaling.","description":"<h4>Background</h4>Amyotrophic lateral sclerosis (ALS) can result in the dysfunction of upper and lower motor neurons. A previous study has indicated that TBK1 mutation (hTBK1-c.978T>A) is involved in progression of ALS. However, the mechanism by which TBK1 mutation mediates the progression of ALS remains unclear.<h4>Methods</h4>NSC-34 cells with hTBK1-c.978T>A mutation (TBK1 mutation status) was used to mimic ALS <i>in vitro</i>. In addition, cell proliferation was detected by Ki67 staining. Gene and protein expressions in NSC-34 cells were detected by RT-qPCR and western blot, respectively. ROS and PGSK levels in NSC-34 cells were detected by flow cytometry.<h4>Results</h4>hTBK1-c.978T>A mutation significantly inhibited the proliferation of NSC-34 cells via inducing cell ferroptosis, whi","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020","modification":"2025-04-18T21:00:26.235Z","creation":"2025-04-07T08:59:18.391Z"},"accession":"S-EPMC7724361","cross_references":{"pubmed":["33312375"]}}