biostudies-literatureUnknown12(11)Zhang Y<h4>Background</h4>Amyotrophic lateral sclerosis (ALS) can result in the dysfunction of upper and lower motor neurons. A previous study has indicated that TBK1 mutation (hTBK1-c.978T>A) is involved in progression of ALS. However, the mechanism by which TBK1 mutation mediates the progression of ALS remains unclear.<h4>Methods</h4>NSC-34 cells with hTBK1-c.978T>A mutation (TBK1 mutation status) was used to mimic ALS <i>in vitro</i>. In addition, cell proliferation was detected by Ki67 staining. Gene and protein expressions in NSC-34 cells were detected by RT-qPCR and western blot, respectively. ROS and PGSK levels in NSC-34 cells were detected by flow cytometry.<h4>Results</h4>hTBK1-c.978T>A mutation significantly inhibited the proliferation of NSC-34 cells via inducing cell ferroptosis, whiAmerican journal of translational research7386-7394https://www.ebi.ac.uk/biostudies/studies/S-EPMC7724361biostudies-literaturehTBK1-c.978T>A mutation promotes the ferroptosis in NSC-34 cells via mediation of KEAP1/NRF2/p62 signaling.PMC7724361Liu XTang LHe JZhang YZhang NFan DfalsehTBK1-c.978T>A mutation promotes the ferroptosis in NSC-34 cells via mediation of KEAP1/NRF2/p62 signaling.<h4>Background</h4>Amyotrophic lateral sclerosis (ALS) can result in the dysfunction of upper and lower motor neurons. A previous study has indicated that TBK1 mutation (hTBK1-c.978T>A) is involved in progression of ALS. However, the mechanism by which TBK1 mutation mediates the progression of ALS remains unclear.<h4>Methods</h4>NSC-34 cells with hTBK1-c.978T>A mutation (TBK1 mutation status) was used to mimic ALS <i>in vitro</i>. In addition, cell proliferation was detected by Ki67 staining. Gene and protein expressions in NSC-34 cells were detected by RT-qPCR and western blot, respectively. ROS and PGSK levels in NSC-34 cells were detected by flow cytometry.<h4>Results</h4>hTBK1-c.978T>A mutation significantly inhibited the proliferation of NSC-34 cells via inducing cell ferroptosis, whi2020-01-01T00:00:00Z20202025-04-18T21:00:26.235Z2025-04-07T08:59:18.391ZS-EPMC772436133312375