biostudies-literatureKnaupp ASARCState Government of VictoriaNational Health and Medical Research CouncilAustralian GovernmentAustralian Research Council1246-1259https://www.ebi.ac.uk/biostudies/studies/S-EPMC7724517biostudies-literatureUnknown15(6)Cellular identity is ultimately dictated by the interaction of transcription factors with regulatory elements (REs) to control gene expression. Advances in epigenome profiling techniques have significantly increased our understanding of cell-specific utilization of REs. However, it remains difficult to dissect the majority of factors that interact with these REs due to the lack of appropriate techniques. Therefore, we developed TINC: TALE-mediated isolation of nuclear chromatin. Using this new method, we interrogated the protein complex formed at the Nanog promoter in embryonic stem cells (ESCs) and identified many known and previously unknown interactors, including RCOR2. Further interrogation of the role of RCOR2 in ESCs revealed its involvement in the repression of lineage genes and theStem cell reportsTINC- A Method to Dissect Regulatory Complexes at Single-Locus Resolution- Reveals an Extensive Protein Complex at the Nanog Promoter.PMC7724517CE140100008APP1069830APP1036587APP1092280GNT1178460FT120100862FT180100674Lim SMPolo JMHuang CFord EChen JNguyen TSchroder JTripathi PMohenska MLister RHolmes MLDavidson KCLarcombe MRLiu XKnaupp ASSun YBYNefzger CMHaigh JJSchittenhelm RBWong KPflueger JFiras JRossello FJDas PPfalseTINC- A Method to Dissect Regulatory Complexes at Single-Locus Resolution- Reveals an Extensive Protein Complex at the Nanog Promoter.Cellular identity is ultimately dictated by the interaction of transcription factors with regulatory elements (REs) to control gene expression. Advances in epigenome profiling techniques have significantly increased our understanding of cell-specific utilization of REs. However, it remains difficult to dissect the majority of factors that interact with these REs due to the lack of appropriate techniques. Therefore, we developed TINC: TALE-mediated isolation of nuclear chromatin. Using this new method, we interrogated the protein complex formed at the Nanog promoter in embryonic stem cells (ESCs) and identified many known and previously unknown interactors, including RCOR2. Further interrogation of the role of RCOR2 in ESCs revealed its involvement in the repression of lineage genes and the2020-01-01T00:00:00Z2020 Dec2026-05-03T04:06:34.986Z2021-02-20T10:55:03ZS-EPMC77245173329667310.1016/j.stemcr.2020.11.005