biostudies-literatureWang XNatural Science Foundation of Jiangxi ProvinceNational Natural Science Foundation of China141https://www.ebi.ac.uk/biostudies/studies/S-EPMC7724852biostudies-literatureUnknown10(1)<h4>Background</h4>Botulinum toxin type A (BTX-A) was considered to be a new potential drug for neuropathic pain (NP) treatment.<h4>Results</h4>In vivo, BTX-A attenuated chronic compression injury (CCI)-induced pain in rats, and reduced production of pro-inflammatory factors. The inhibition of BTX-A to expression and phosphorylation of SNAP23 were partly reversed by TLR2/MyD88 upregulation. In LPS-stimulated microglia, we also found that BTX-A suppressed TLR2, MyD88, p-SNAP23 and SNAP23 expression, and reduced pro-inflammatory factors secretion. Upregulation of TLR2 and MyD88 recued the inhibition of BTX-A to LPS-induced activation of SNAP23. Then, we demonstrated that BTX-A reduced expression of SNAP23 through inhibition of IKKα/β phosphorylation. Besides, the inhibition of BTX-A to LPS-iCell & bioscienceBotulinum toxin type A alleviates neuropathic pain and suppresses inflammatory cytokines release from microglia by targeting TLR2/MyD88 and SNAP23.PMC772485220192BAB20504731860293Zheng HWu LLiu PTian SLiu QWang XWang HWu WfalseBotulinum toxin type A alleviates neuropathic pain and suppresses inflammatory cytokines release from microglia by targeting TLR2/MyD88 and SNAP23.<h4>Background</h4>Botulinum toxin type A (BTX-A) was considered to be a new potential drug for neuropathic pain (NP) treatment.<h4>Results</h4>In vivo, BTX-A attenuated chronic compression injury (CCI)-induced pain in rats, and reduced production of pro-inflammatory factors. The inhibition of BTX-A to expression and phosphorylation of SNAP23 were partly reversed by TLR2/MyD88 upregulation. In LPS-stimulated microglia, we also found that BTX-A suppressed TLR2, MyD88, p-SNAP23 and SNAP23 expression, and reduced pro-inflammatory factors secretion. Upregulation of TLR2 and MyD88 recued the inhibition of BTX-A to LPS-induced activation of SNAP23. Then, we demonstrated that BTX-A reduced expression of SNAP23 through inhibition of IKKα/β phosphorylation. Besides, the inhibition of BTX-A to LPS-i2020-01-01T00:00:00Z2020 Dec2025-04-27T02:58:23.859Z2025-04-06T18:39:08.953ZS-EPMC77248523329817110.1186/s13578-020-00501-4