biostudies-literatureBetari NStiftelsen Kristian Gerhard JebsenNorges ForskningsrådHorizon 2020 Framework ProgrammeKnut och Alice Wallenbergs StiftelseFederación Española de Enfermedades Raras593416https://www.ebi.ac.uk/biostudies/studies/S-EPMC7726444biostudies-literatureUnknown11Serotonin (5-HT) is a hormone and neurotransmitter that modulates neural activity as well as a wide range of other physiological processes including cardiovascular function, bowel motility, and platelet aggregation. 5-HT synthesis is catalyzed by tryptophan hydroxylase (TPH) which exists as two distinct isoforms; TPH1 and TPH2, which are responsible for peripheral and central 5-HT, respectively. Due to the implication of 5-HT in a number of pathologies, including depression, anxiety, autism, sexual dysfunction, irritable bowel syndrome, inflammatory bowel disease, and carcinoid syndrome, there has been a growing interest in finding modulators of these enzymes in recent years. We thus performed high-throughput screening (HTS) using a fluorescence-based thermal shift assay (DSF) to search thFrontiers in pharmacologyInhibition of Tryptophan Hydroxylases and Monoamine Oxidase-A by the Proton Pump Inhibitor, Omeprazole-<i>In Vitro</i> and <i>In Vivo</i> Investigations.PMC7726444501100005416667302SKGJ-MED02SAF2017-87349-R501100004063Godoy-Marin HBetari NCiruela FMorato XSahlholm KTeigen KJauregui OHaavik JfalseInhibition of Tryptophan Hydroxylases and Monoamine Oxidase-A by the Proton Pump Inhibitor, Omeprazole-<i>In Vitro</i> and <i>In Vivo</i> Investigations.Serotonin (5-HT) is a hormone and neurotransmitter that modulates neural activity as well as a wide range of other physiological processes including cardiovascular function, bowel motility, and platelet aggregation. 5-HT synthesis is catalyzed by tryptophan hydroxylase (TPH) which exists as two distinct isoforms; TPH1 and TPH2, which are responsible for peripheral and central 5-HT, respectively. Due to the implication of 5-HT in a number of pathologies, including depression, anxiety, autism, sexual dysfunction, irritable bowel syndrome, inflammatory bowel disease, and carcinoid syndrome, there has been a growing interest in finding modulators of these enzymes in recent years. We thus performed high-throughput screening (HTS) using a fluorescence-based thermal shift assay (DSF) to search th2020-01-01T00:00:00Z20202026-05-02T06:31:05.827Z2021-02-20T16:45:59ZS-EPMC77264443332422110.3389/fphar.2020.593416