{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["16(11)"],"submitter":["Godot V"],"pubmed_abstract":["The development of HIV-1 vaccines is challenged by the lack of relevant models to accurately induce human B- and T-cell responses in lymphoid organs. In humanized mice reconstituted with human hematopoietic stem cells (hu-mice), human B cell-development and function are impaired and cells fail to efficiently transition from IgM B cells to IgG B cells. Here, we found that CD40-targeted vaccination combined with CpG-B adjuvant overcomes the usual defect of human B-cell switch and maturation in hu-mice. We further dissected hu-B cell responses directed against the HIV-1 Env protein elicited by targeting Env gp140 clade C to the CD40 receptor of antigen-presenting cells. The anti-CD40.Env gp140 vaccine was injected with CpG-B in a homologous prime/boost regimen or as a boost of a NYVAC-KC pox "],"journal":["PLoS pathogens"],"pagination":["e1009025"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7728200"],"repository":["biostudies-literature"],"pubmed_title":["TLR-9 agonist and CD40-targeting vaccination induces HIV-1 envelope-specific B cells with a diversified immunoglobulin repertoire in humanized mice."],"pmcid":["PMC7728200"],"pubmed_authors":["Li G","Cardinaud S","Levy Y","Su L","Mouquet H","Fenwick C","Godot V","Pantaleo G","Gil L","Zurawski SM","Tcherakian C","Ortonne N","Centlivre M","Cheng L","Zurawski G","Cervera-Marzal I","Lelievre JD","Milpied P"],"additional_accession":[]},"is_claimable":false,"name":"TLR-9 agonist and CD40-targeting vaccination induces HIV-1 envelope-specific B cells with a diversified immunoglobulin repertoire in humanized mice.","description":"The development of HIV-1 vaccines is challenged by the lack of relevant models to accurately induce human B- and T-cell responses in lymphoid organs. In humanized mice reconstituted with human hematopoietic stem cells (hu-mice), human B cell-development and function are impaired and cells fail to efficiently transition from IgM B cells to IgG B cells. Here, we found that CD40-targeted vaccination combined with CpG-B adjuvant overcomes the usual defect of human B-cell switch and maturation in hu-mice. We further dissected hu-B cell responses directed against the HIV-1 Env protein elicited by targeting Env gp140 clade C to the CD40 receptor of antigen-presenting cells. The anti-CD40.Env gp140 vaccine was injected with CpG-B in a homologous prime/boost regimen or as a boost of a NYVAC-KC pox ","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Nov","modification":"2026-04-07T21:47:18.794Z","creation":"2021-02-20T12:54:39Z"},"accession":"S-EPMC7728200","cross_references":{"pubmed":["33253297"],"doi":["10.1371/journal.ppat.1009025"]}}