biostudies-literatureYou SKey R&D Program of Shandong Province2147367https://www.ebi.ac.uk/biostudies/studies/S-EPMC7732375biostudies-literatureUnknown2020Ammonium (NH4 +) plays key roles in plant growth, development, fruit quality, and yield. In plants, NH4 + uptake and transport are facilitated by NH4 + transporters (AMT). However, molecular mechanisms and physiological functions of type-II AMT (AMT2) transporters in fruit trees are still unclear, especially in peach. In this study, we cloned and characterized an AMT2 family gene from peach, PpeAMT3;4, and determined its function in yeast mutant. Expression analysis showed that PpeAMT3;4 was majorly expressed in peach roots and significantly decreased by NH4 + excess but had no response to NH4 + deficiency. Functional determination and 15nitrogen-labeled NH4 + uptake assay in yeast cells implied that PpeAMT3;4 was a typical high-affinity transporter, with a K m value of 86.3 μM, that can uptake external NH4 + in yeast cells. This study provides gene resources to uncover the biological function of AMT2 transporters and reveals molecular basis for NH4 + uptake and nitrogen (N) nutrition mechanisms in fruit trees.BioMed research internationalCloning and Functional Determination of Ammonium Transporter PpeAMT3;4 in Peach.PMC7732375cstc2019jscx-gksbX0138NKY201900302018JHZ0062019YFD10005002019LZGC009cstc2019jxjl800122019GSF107095Wu ZLi YYou SShi WWang YTan PSong ZfalseCloning and Functional Determination of Ammonium Transporter PpeAMT3;4 in Peach.Ammonium (NH4 +) plays key roles in plant growth, development, fruit quality, and yield. In plants, NH4 + uptake and transport are facilitated by NH4 + transporters (AMT). However, molecular mechanisms and physiological functions of type-II AMT (AMT2) transporters in fruit trees are still unclear, especially in peach. In this study, we cloned and characterized an AMT2 family gene from peach, PpeAMT3;4, and determined its function in yeast mutant. Expression analysis showed that PpeAMT3;4 was majorly expressed in peach roots and significantly decreased by NH4 + excess but had no response to NH4 + deficiency. Functional determination and 15nitrogen-labeled NH4 + uptake assay in yeast cells implied that PpeAMT3;4 was a typical high-affinity transporter, with a K m value of 86.3 μM, that can uptake external NH4 + in yeast cells. This study provides gene resources to uncover the biological function of AMT2 transporters and reveals molecular basis for NH4 + uptake and nitrogen (N) nutrition mechanisms in fruit trees.2020-01-01T00:00:00Z20202024-11-15T16:35:11.299Z2021-02-20T16:45:52ZS-EPMC77323753334463110.1155/2020/2147367