biostudies-literatureKuroda KKAKENHI4500-4509https://www.ebi.ac.uk/biostudies/studies/S-EPMC7734156biostudies-literatureUnknown111(12)Fibroblast growth factor receptor (FGFR) is associated with proliferation, migration, and angiogenesis of carcinomas, and FGFR signaling inhibitors are considered a key drug for the treatment of solid tumors with FGFR overexpression. Amplification of FGFR2 is reportedly identified in 3%-10% of gastric cancers (GCs). The aim of this study is to clarify whether the identification of the circulating tumor cells (CTCs) with FGFR2 overexpression is useful to detect patients with FGFR2-overexpressing GC. One hundred GC patients who underwent gastrectomy were enrolled. A total volume of 8 mL of peripheral blood was collected from each patient just before gastrectomy, and mononuclear cells were enriched by Ficol density gradient centrifugation. These cells were immunostained with PI/CD45/EpCAM/FGFCancer scienceCirculating tumor cells with FGFR2 expression might be useful to identify patients with existing FGFR2-overexpressing tumor.PMC77341562339032918H02883Sugimoto AKushiyama SSera TYamamoto YYashiro MNishimura SOkuno TKuroda KMiki YTogano SOhira MfalseCirculating tumor cells with FGFR2 expression might be useful to identify patients with existing FGFR2-overexpressing tumor.Fibroblast growth factor receptor (FGFR) is associated with proliferation, migration, and angiogenesis of carcinomas, and FGFR signaling inhibitors are considered a key drug for the treatment of solid tumors with FGFR overexpression. Amplification of FGFR2 is reportedly identified in 3%-10% of gastric cancers (GCs). The aim of this study is to clarify whether the identification of the circulating tumor cells (CTCs) with FGFR2 overexpression is useful to detect patients with FGFR2-overexpressing GC. One hundred GC patients who underwent gastrectomy were enrolled. A total volume of 8 mL of peripheral blood was collected from each patient just before gastrectomy, and mononuclear cells were enriched by Ficol density gradient centrifugation. These cells were immunostained with PI/CD45/EpCAM/FGF2020-01-01T00:00:00Z2020 Dec2025-05-31T23:54:05.019Z2021-02-20T14:29:37ZS-EPMC77341563294665510.1111/cas.14654