{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kalinski AL"],"funding":["Dr. Miriam and Sheldon G. Adelson Medical Research Foundation","NEI NIH HHS","Wings for Life","New York State Department of Health","NINDS NIH HHS","National Institutes of Health","Stanley D. and Joan H. Ross Chair in Neuromodulation fund","National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["e60223"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7735761"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9"],"pubmed_abstract":["Sciatic nerve crush injury triggers sterile inflammation within the distal nerve and axotomized dorsal root ganglia (DRGs). Granulocytes and pro-inflammatory Ly6C<sup>high</sup> monocytes infiltrate the nerve first and rapidly give way to Ly6C<sup>negative</sup> inflammation-resolving macrophages. In axotomized DRGs, few hematogenous leukocytes are detected and resident macrophages acquire a ramified morphology. Single-cell RNA-sequencing of injured sciatic nerve identifies five macrophage subpopulations, repair Schwann cells, and mesenchymal precursor cells. Macrophages at the nerve crush site are molecularly distinct from macrophages associated with Wallerian degeneration. In the injured nerve, macrophages 'eat' apoptotic leukocytes, a process called efferocytosis, and thereby promote an"],"journal":["eLife"],"pubmed_title":["Analysis of the immune response to sciatic nerve injury identifies efferocytosis as a key mechanism of nerve debridement."],"pmcid":["PMC7735761"],"funding_grant_id":["R01EY028350","T32-GM113900","T32 GM113900","Program","T32-NS07222","R01EY029159","T32 NS007222","R01 EY029159","endowment","fellowship","R01 EY028350","C33267GG"],"pubmed_authors":["Huffman LD","Hollis E","Jara JS","Carbajal KS","Segal BM","Kawaguchi R","Hafner H","Duncker PC","Giger RJ","Passino R","Yoon C","Kalinski AL","Kohen R","Johnson C","Geschwind DH"],"additional_accession":[]},"is_claimable":false,"name":"Analysis of the immune response to sciatic nerve injury identifies efferocytosis as a key mechanism of nerve debridement.","description":"Sciatic nerve crush injury triggers sterile inflammation within the distal nerve and axotomized dorsal root ganglia (DRGs). Granulocytes and pro-inflammatory Ly6C<sup>high</sup> monocytes infiltrate the nerve first and rapidly give way to Ly6C<sup>negative</sup> inflammation-resolving macrophages. In axotomized DRGs, few hematogenous leukocytes are detected and resident macrophages acquire a ramified morphology. Single-cell RNA-sequencing of injured sciatic nerve identifies five macrophage subpopulations, repair Schwann cells, and mesenchymal precursor cells. Macrophages at the nerve crush site are molecularly distinct from macrophages associated with Wallerian degeneration. In the injured nerve, macrophages 'eat' apoptotic leukocytes, a process called efferocytosis, and thereby promote an","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Dec","modification":"2026-05-01T10:00:28.423Z","creation":"2021-02-20T16:51:57Z"},"accession":"S-EPMC7735761","cross_references":{"pubmed":["33263277"],"doi":["10.7554/eLife.60223"]}}