biostudies-literatureSun WMinistry of Science and Technology of the People's Republic of ChinaMinistry of Education of the People's Republic of ChinaNational Natural Science Foundation of ChinaBeijing Municipal Commission of EducationDezhou University31700-31705https://www.ebi.ac.uk/biostudies/studies/S-EPMC7745405biostudies-literatureUnknown5(49)Fluorescence detection is desirable to track the gene transfer process in order to explain the mechanism. Here, a fluorescent nanoparticle, diketopyrrolopyrrole-based liposome (DPL), was prepared for DNA delivery and tumor imaging in vitro and in vivo. The process to deliver DNA into cells was detected in real time by DPL according to the fluorescent property. The transfection efficacies (TEs) for luciferase and enhanced green fluorescent protein (EGFP) analysis of DPL were 1.5 times those of the commercial transfection agent Lipo 2000. Importantly, the DPL/DNA system has high EGFP TE in vivo with tumor targeting ability. This work provided an effective strategy for monitoring transfection processes.ACS omegaFluorescent Nanoparticles for Targeted Tumor Imaging and DNA Tracking Gene Delivery In Vitro/In Vivo.PMC7745405213720322019xjrc3352016YFE0205400-00521778012Cui JXSun WTang FLu ZLfalseFluorescent Nanoparticles for Targeted Tumor Imaging and DNA Tracking Gene Delivery In Vitro/In Vivo.Fluorescence detection is desirable to track the gene transfer process in order to explain the mechanism. Here, a fluorescent nanoparticle, diketopyrrolopyrrole-based liposome (DPL), was prepared for DNA delivery and tumor imaging in vitro and in vivo. The process to deliver DNA into cells was detected in real time by DPL according to the fluorescent property. The transfection efficacies (TEs) for luciferase and enhanced green fluorescent protein (EGFP) analysis of DPL were 1.5 times those of the commercial transfection agent Lipo 2000. Importantly, the DPL/DNA system has high EGFP TE in vivo with tumor targeting ability. This work provided an effective strategy for monitoring transfection processes.2020-01-01T00:00:00Z2020 Dec2025-04-26T09:06:27.562Z2021-02-20T16:38:31ZS-EPMC77454053334482210.1021/acsomega.0c04213