biostudies-literatureHeissenberger CHochschuljubiläumsstiftung der Stadt WienAustrian Science Fund FWFMount Desert Island Biological LaboratoryUniversité Libre de BruxellesBelgian National Fund for Scientific ResearchRégion Wallonne (DGO6)Région WallonneNational Institute of General Medical SciencesNIGMS NIH HHSNIH HHSHerzfelder'sche Familienstiftunge56205https://www.ebi.ac.uk/biostudies/studies/S-EPMC7746234biostudies-literatureUnknown9Our knowledge about the repertoire of ribosomal RNA modifications and the enzymes responsible for installing them is constantly expanding. Previously, we reported that NSUN-5 is responsible for depositing m5C at position C2381 on the 26S rRNA in Caenorhabditis elegans. Here, we show that NSUN-1 is writing the second known 26S rRNA m5C at position C2982. Depletion of nsun-1 or nsun-5 improved thermotolerance and slightly increased locomotion at midlife, however, only soma-specific knockdown of nsun-1 extended lifespan. Moreover, soma-specific knockdown of nsun-1 reduced body size and impaired fecundity, suggesting non-cell-autonomous effects. While ribosome biogenesis and global protein synthesis were unaffected by nsun-1 depleeLifeThe ribosomal RNA m5C methyltransferase NSUN-1 modulates healthspan and oogenesis in Caenorhabditis elegans.PMC7746234H- 327123/2018F.R.S./FNRSP30623W 1224P20GM103423grant RIBO cancer no. 18100701810070P20GM104318P40 OD010440James L. Boyer FellowshipP20 GM104318I2514P20 GM103423P 30623W1224Stocker IWacheul LRollins JAKrammer TLGrillari JSchosserer MSnow SRogers ANHeissenberger CLafontaine DLJTav KShpylovyi ANagelreiter FfalseThe ribosomal RNA m5C methyltransferase NSUN-1 modulates healthspan and oogenesis in Caenorhabditis elegans.Our knowledge about the repertoire of ribosomal RNA modifications and the enzymes responsible for installing them is constantly expanding. Previously, we reported that NSUN-5 is responsible for depositing m5C at position C2381 on the 26S rRNA in Caenorhabditis elegans. Here, we show that NSUN-1 is writing the second known 26S rRNA m5C at position C2982. Depletion of nsun-1 or nsun-5 improved thermotolerance and slightly increased locomotion at midlife, however, only soma-specific knockdown of nsun-1 extended lifespan. Moreover, soma-specific knockdown of nsun-1 reduced body size and impaired fecundity, suggesting non-cell-autonomous effects. While ribosome biogenesis and global protein synthesis were unaffected by nsun-1 deple2020-01-01T00:00:00Z2020 Dec2026-04-29T20:21:01.271Z2021-02-20T15:41:40ZS-EPMC77462343328948010.7554/eLife.56205