{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["10(1)"],"submitter":["Dinkar V"],"pubmed_abstract":["TSD276-2, a wheat genetic stock derived from the cross Agra Local/T. spelta 276 showed broad spectrum resistance against leaf rust pathogen. Genetic analysis was undertaken using F<sub>1</sub>, F<sub>2</sub>, F<sub>2:3</sub> and BC<sub>1</sub>F<sub>1</sub> generations derived from the cross TSD276-2/Agra Local. The results revealed a single recessive gene for leaf rust resistance, tentatively named as LrTs<sub>276-2,</sub> in TSD276-2. Molecular mapping of leaf rust resistance gene LrTs<sub>276-2</sub> in TSD276-2 was done using SNP-based PCR and SSR markers. For Bulked Segregant Analysis (BSA), two bulks viz. resistant bulk and susceptible bulk, and the parents TSD276-2 and Agra Local were genotyped for SNPs using AFFYMETRIX 35K Wheat Breeders' AXIOM array. T. spelta 276 was also genotyped and used as a check. BSA indicated that the gene for leaf rust resistance in TSD276-2 is located on chromosome arm 1DS. Putatively linked SNPs on chromosome arm 1DS were converted into PCR-based markers. Polymorphic SSR markers on chromosome arm 1DS were also identified. Final linkage map was constructed using one SNP-based PCR and three SSR markers. The rust reaction and chromosomal location suggest that LrTs<sub>276-2</sub> is a new leaf rust resistance gene which may be useful in broadening the genetic base of leaf rust resistance in wheat."],"journal":["Scientific reports"],"pagination":["22113"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7746701"],"repository":["biostudies-literature"],"pubmed_title":["Molecular mapping of a new recessive wheat leaf rust resistance gene originating from Triticum spelta."],"pmcid":["PMC7746701"],"pubmed_authors":["Mallick N","Agarwal P","Sharma JB","Jha SK","Niranjana M","Dinkar V","Vinod"],"additional_accession":[]},"is_claimable":false,"name":"Molecular mapping of a new recessive wheat leaf rust resistance gene originating from Triticum spelta.","description":"TSD276-2, a wheat genetic stock derived from the cross Agra Local/T. spelta 276 showed broad spectrum resistance against leaf rust pathogen. Genetic analysis was undertaken using F<sub>1</sub>, F<sub>2</sub>, F<sub>2:3</sub> and BC<sub>1</sub>F<sub>1</sub> generations derived from the cross TSD276-2/Agra Local. The results revealed a single recessive gene for leaf rust resistance, tentatively named as LrTs<sub>276-2,</sub> in TSD276-2. Molecular mapping of leaf rust resistance gene LrTs<sub>276-2</sub> in TSD276-2 was done using SNP-based PCR and SSR markers. For Bulked Segregant Analysis (BSA), two bulks viz. resistant bulk and susceptible bulk, and the parents TSD276-2 and Agra Local were genotyped for SNPs using AFFYMETRIX 35K Wheat Breeders' AXIOM array. T. spelta 276 was also genotyped and used as a check. BSA indicated that the gene for leaf rust resistance in TSD276-2 is located on chromosome arm 1DS. Putatively linked SNPs on chromosome arm 1DS were converted into PCR-based markers. Polymorphic SSR markers on chromosome arm 1DS were also identified. Final linkage map was constructed using one SNP-based PCR and three SSR markers. The rust reaction and chromosomal location suggest that LrTs<sub>276-2</sub> is a new leaf rust resistance gene which may be useful in broadening the genetic base of leaf rust resistance in wheat.","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Dec","modification":"2024-02-15T19:10:39.26Z","creation":"2021-02-20T13:41:57Z"},"accession":"S-EPMC7746701","cross_references":{"pubmed":["33335131"],"doi":["10.1038/s41598-020-78679-3"]}}