{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sparks H"],"funding":["Cancer Research UK","Francis Crick Institute","The Francis Crick Institute","Engineering and Physical Sciences","Wellcome Trust","Engineering and Physical Sciences Research Council"],"pagination":["7204-7220"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7747899"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(12)"],"pubmed_abstract":["We present a new folded dual-view oblique plane microscopy (OPM) technique termed dOPM that enables two orthogonal views of the sample to be obtained by translating a pair of tilted mirrors in refocussing space. Using a water immersion 40× 1.15 NA primary objective, deconvolved image volumes of 200 nm beads were measured to have full width at half maxima (FWHM) of 0.35 ± 0.04 µm and 0.39 ± 0.02 µm laterally and 0.81 ± 0.07 µm axially. The measured z-sectioning value was 1.33 ± 0.45 µm using light-sheet FWHM in the frames of the two views of 4.99 ± 0.58 µm and 4.89 ± 0.63 µm. To qualitatively demonstrate that the system can reduce shadow artefacts while providing a more isotropic resolution, a multi-cellular spheroid approximately 100 µm in diameter was imaged."],"journal":["Biomedical optics express"],"pubmed_title":["Dual-view oblique plane microscopy (dOPM)."],"pmcid":["PMC7747899"],"funding_grant_id":["FC001317","20146","10317","FC001039","21144","C37275/A20146","EP/T003103/1","10039"],"pubmed_authors":["Behrens A","Dunsby C","Sparks H","Bakal C","Dent L","Salbreux G"],"additional_accession":[]},"is_claimable":false,"name":"Dual-view oblique plane microscopy (dOPM).","description":"We present a new folded dual-view oblique plane microscopy (OPM) technique termed dOPM that enables two orthogonal views of the sample to be obtained by translating a pair of tilted mirrors in refocussing space. Using a water immersion 40× 1.15 NA primary objective, deconvolved image volumes of 200 nm beads were measured to have full width at half maxima (FWHM) of 0.35 ± 0.04 µm and 0.39 ± 0.02 µm laterally and 0.81 ± 0.07 µm axially. The measured z-sectioning value was 1.33 ± 0.45 µm using light-sheet FWHM in the frames of the two views of 4.99 ± 0.58 µm and 4.89 ± 0.63 µm. To qualitatively demonstrate that the system can reduce shadow artefacts while providing a more isotropic resolution, a multi-cellular spheroid approximately 100 µm in diameter was imaged.","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Dec","modification":"2026-07-09T11:05:41.205Z","creation":"2026-07-09T10:37:42.272Z"},"accession":"S-EPMC7747899","cross_references":{"pubmed":["33408991"],"doi":["10.1364/BOE.409781"]}}