biostudies-literatureSparks HCancer Research UKFrancis Crick InstituteThe Francis Crick InstituteEngineering and Physical SciencesWellcome TrustEngineering and Physical Sciences Research Council7204-7220https://www.ebi.ac.uk/biostudies/studies/S-EPMC7747899biostudies-literatureUnknown11(12)We present a new folded dual-view oblique plane microscopy (OPM) technique termed dOPM that enables two orthogonal views of the sample to be obtained by translating a pair of tilted mirrors in refocussing space. Using a water immersion 40× 1.15 NA primary objective, deconvolved image volumes of 200 nm beads were measured to have full width at half maxima (FWHM) of 0.35 ± 0.04 µm and 0.39 ± 0.02 µm laterally and 0.81 ± 0.07 µm axially. The measured z-sectioning value was 1.33 ± 0.45 µm using light-sheet FWHM in the frames of the two views of 4.99 ± 0.58 µm and 4.89 ± 0.63 µm. To qualitatively demonstrate that the system can reduce shadow artefacts while providing a more isotropic resolution, a multi-cellular spheroid approximately 100 µm in diameter was imaged.Biomedical optics expressDual-view oblique plane microscopy (dOPM).PMC7747899FC0013172014610317FC00103921144C37275/A20146EP/T003103/110039Behrens ADunsby CSparks HBakal CDent LSalbreux GfalseDual-view oblique plane microscopy (dOPM).We present a new folded dual-view oblique plane microscopy (OPM) technique termed dOPM that enables two orthogonal views of the sample to be obtained by translating a pair of tilted mirrors in refocussing space. Using a water immersion 40× 1.15 NA primary objective, deconvolved image volumes of 200 nm beads were measured to have full width at half maxima (FWHM) of 0.35 ± 0.04 µm and 0.39 ± 0.02 µm laterally and 0.81 ± 0.07 µm axially. The measured z-sectioning value was 1.33 ± 0.45 µm using light-sheet FWHM in the frames of the two views of 4.99 ± 0.58 µm and 4.89 ± 0.63 µm. To qualitatively demonstrate that the system can reduce shadow artefacts while providing a more isotropic resolution, a multi-cellular spheroid approximately 100 µm in diameter was imaged.2020-01-01T00:00:00Z2020 Dec2026-07-09T11:05:41.205Z2026-07-09T10:37:42.272ZS-EPMC77478993340899110.1364/BOE.409781