{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Dixit UG"],"funding":["American Heart Association","BLRD VA","FIC NIH HHS","NIAID NIH HHS","Department of Veterans’ Affairs","University of Northern Iowa College of Humanities, Arts and Sciences","U. S. National Institutes of Health"],"pagination":["107968"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7750307"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["220"],"pubmed_abstract":["The parasitic protozoan Leishmania infantum resides primarily in macrophages throughout mammalian infection. Infection is initiated by deposition of the metacyclic promastigote into the dermis of a mammalian host by the sand fly vector. Promastigotes enter macrophages by ligating surface receptors such as complement receptor 3 (CR3), inducing phagocytosis of the parasite. At the binding site of metacyclic promastigotes, we observed large asymmetrical aggregates of macrophage membrane with underlying actin, resembling membrane ruffles. Actin accumulation was observed at the point of initial contact, before phagosome formation and accumulation of peri-phagosomal actin. Ruffle-like structures did not form during phagocytosis of attenuated promastigotes or during phagocytosis of the intracellu"],"journal":["Experimental parasitology"],"pubmed_title":["Complement receptor 3 mediates ruffle-like, actin-rich aggregates during phagocytosis of Leishmania infantum metacyclics."],"pmcid":["PMC7750307"],"funding_grant_id":["R01 TW010500","R01 AI056242","I01 BX001983","R01 AI045540","R01AI056242","AI045540","R01 TW 010500","T32AI007511","T32 AI007511","0435333Z","I01 BX000536"],"pubmed_authors":["Rodriguez NE","Dixit UG","McDowell MA","Wilson ME","Polando R"],"additional_accession":[]},"is_claimable":false,"name":"Complement receptor 3 mediates ruffle-like, actin-rich aggregates during phagocytosis of Leishmania infantum metacyclics.","description":"The parasitic protozoan Leishmania infantum resides primarily in macrophages throughout mammalian infection. Infection is initiated by deposition of the metacyclic promastigote into the dermis of a mammalian host by the sand fly vector. Promastigotes enter macrophages by ligating surface receptors such as complement receptor 3 (CR3), inducing phagocytosis of the parasite. At the binding site of metacyclic promastigotes, we observed large asymmetrical aggregates of macrophage membrane with underlying actin, resembling membrane ruffles. Actin accumulation was observed at the point of initial contact, before phagosome formation and accumulation of peri-phagosomal actin. Ruffle-like structures did not form during phagocytosis of attenuated promastigotes or during phagocytosis of the intracellu","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jan","modification":"2025-04-21T21:39:41.215Z","creation":"2022-02-11T13:38:56.98Z"},"accession":"S-EPMC7750307","cross_references":{"pubmed":["32781093"],"doi":["10.1016/j.exppara.2020.107968"]}}