biostudies-literatureKim HMRural Development AdministrationNational Research Foundation of KoreaChonnam National University583767https://www.ebi.ac.uk/biostudies/studies/S-EPMC7752897biostudies-literatureUnknown11Abscisic acid (ABA) is a key signaling molecule that mediates plant response to stress. Increasing evidence indicates that ABA also regulates many aspects of plant development, such as seed germination, leaf development, and ripening. ABA metabolism, including ABA biosynthesis and degradation, is an essential aspect of ABA response in plants. In this study, we identified four cytochrome P450 genes (<i>CaCYP707A1</i>, <i>2</i>, <i>3</i>, and <i>4</i>) that mediate ABA hydroxylation, which is required for ABA degradation in <i>Capsicum annuum</i>. We observed that CaCYP707A-mediated ABA hydroxylation promotes ABA degradation, leading to low levels of ABA and a dehydration phenotype in <i>35S:CaCYP707A</i> plants. Importantly, seed formation was strongly inhibited in <i>35S:CaCYP707A</i> planFrontiers in plant sciencePromoted ABA Hydroxylation by <i>Capsicum annuum CYP707As</i> Overexpression Suppresses Pollen Maturation in <i>Nicotiana tabacum</i>.PMC7752897Grant No. 2020-1850Project No. PJ014874012018R1A4A1023882Yun CHKim HMJang GMa SHJoung YHPark SHPark SYfalsePromoted ABA Hydroxylation by <i>Capsicum annuum CYP707As</i> Overexpression Suppresses Pollen Maturation in <i>Nicotiana tabacum</i>.Abscisic acid (ABA) is a key signaling molecule that mediates plant response to stress. Increasing evidence indicates that ABA also regulates many aspects of plant development, such as seed germination, leaf development, and ripening. ABA metabolism, including ABA biosynthesis and degradation, is an essential aspect of ABA response in plants. In this study, we identified four cytochrome P450 genes (<i>CaCYP707A1</i>, <i>2</i>, <i>3</i>, and <i>4</i>) that mediate ABA hydroxylation, which is required for ABA degradation in <i>Capsicum annuum</i>. We observed that CaCYP707A-mediated ABA hydroxylation promotes ABA degradation, leading to low levels of ABA and a dehydration phenotype in <i>35S:CaCYP707A</i> plants. Importantly, seed formation was strongly inhibited in <i>35S:CaCYP707A</i> plan2020-01-01T00:00:00Z20202026-04-28T22:53:16.251Z2021-02-20T17:35:57ZS-EPMC77528973336355310.3389/fpls.2020.583767