biostudies-literatureGou GMINECONIMH NIH HHSNINDS NIH HHSCareer Integration GrantMinisterio de Economía y CompetitividadNational Institutes of HealthAGAURRamón y Cajal FellowshipNIH HHS618-634https://www.ebi.ac.uk/biostudies/studies/S-EPMC7754318biostudies-literatureUnknown154(6)The SynGAP protein is a major regulator of synapse biology and neural circuit function. Genetic variants linked to epilepsy and intellectual disability disrupt synaptic function and neural excitability. SynGAP has been involved in multiple signaling pathways and can regulate small GTPases with very different roles. Yet, the molecular bases behind this pleiotropy are poorly understood. We hypothesize that different SynGAP isoforms will mediate different sets of functions and that deciphering their spatio-temporal expression and subcellular localization will accelerate understanding their multiple functions. Using isoform-specific antibodies recognizing SynGAP in mouse and human samples we found distinctive developmental expression patterns for all SynGAP isoforms in five mouse brain areas. Journal of neurochemistrySynGAP splice variants display heterogeneous spatio-temporal expression and subcellular distribution in the developing mammalian brain.PMC7754318R01 MH112151R01 MH113648MH096847R01 NS064079R37 NS036715BFU2012-343982017SGR1776IEDI‐2017‐00822R01 NS110307R01 MH096847NS064079SGR14-297R01 MH108408RTI2018‐097037‐B‐100RO1 MH112151BFU2012‐34398MH108408R01 NS036715BFU2015-69717-PRYC-2011-08391pRTI2018-097037-B-100BFU2015‐69717‐P304111BES‐2013‐063720BES-2013-063720IEDI-2017-00822Roca-Fernandez AKilinc MAraki YRumbaugh GBayes ASerrano EGou GReig-Viader Rde Quintana-Schmidt CHuganir RLfalseSynGAP splice variants display heterogeneous spatio-temporal expression and subcellular distribution in the developing mammalian brain.The SynGAP protein is a major regulator of synapse biology and neural circuit function. Genetic variants linked to epilepsy and intellectual disability disrupt synaptic function and neural excitability. SynGAP has been involved in multiple signaling pathways and can regulate small GTPases with very different roles. Yet, the molecular bases behind this pleiotropy are poorly understood. We hypothesize that different SynGAP isoforms will mediate different sets of functions and that deciphering their spatio-temporal expression and subcellular localization will accelerate understanding their multiple functions. Using isoform-specific antibodies recognizing SynGAP in mouse and human samples we found distinctive developmental expression patterns for all SynGAP isoforms in five mouse brain areas. 2020-01-01T00:00:00Z2020 Sep2026-05-03T19:44:03.12Z2021-02-20T15:52:20ZS-EPMC77543183206825210.1111/jnc.14988