{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Findlay J"],"funding":["MRF","UKRI | MRC | Medical Research Foundation","UKRI | Biotechnology and Biological Sciences Research Council","Medical Research Foundation","UKRI | Natural Environment Research Council","Natural Environment Research Council","Biotechnology and Biological Sciences Research Council"],"pagination":["e01842-20"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7755243"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["87(1)"],"pubmed_abstract":["Third-generation cephalosporin resistance (3GC-R) in <i>Escherichia coli</i> is a rising problem in human and farmed-animal populations. We conducted whole-genome sequencing analysis of 138 representative 3GC-R isolates previously collected from dairy farms in southwest England and confirmed by PCR to carry acquired 3GC-R genes. This analysis identified <i>bla</i> <sub>CTX-M</sub> (131 isolates encoding CTX-M-1, -14, -15, -and 32 and the novel variant CTX-M-214), <i>bla</i> <sub>CMY-2</sub> (6 isolates), and <i>bla</i> <sub>DHA-1</sub> (1 isolate). A highly conserved plasmid was identified in 73 isolates, representing 27 <i>E. coli</i> sequence types. This novel ∼220-kb IncHI2 plasmid carrying <i>bla</i> <sub>CTX-M-32</sub> was sequenced to closure and designated pMOO-32. It was found expe"],"journal":["Applied and environmental microbiology"],"pubmed_title":["Molecular Epidemiology of Escherichia coli Producing CTX-M and pAmpC β-Lactamases from Dairy Farms Identifies a Dominant Plasmid Encoding CTX-M-32 but No Evidence for Transmission to Humans in the Same Geographical Region."],"pmcid":["PMC7755243"],"funding_grant_id":["MRF_MRF-145-0004-TPG-AVISO","NE/N01961X/1","BB/T004592/1","MRF-145-0004-TPG-AVISO"],"pubmed_authors":["Mounsey O","Avison MB","Morley K","Schubert H","Findlay J","Gould VC","Lee WWY","Cogan TA","Newbold N","Reyher KK"],"additional_accession":[]},"is_claimable":false,"name":"Molecular Epidemiology of Escherichia coli Producing CTX-M and pAmpC β-Lactamases from Dairy Farms Identifies a Dominant Plasmid Encoding CTX-M-32 but No Evidence for Transmission to Humans in the Same Geographical Region.","description":"Third-generation cephalosporin resistance (3GC-R) in <i>Escherichia coli</i> is a rising problem in human and farmed-animal populations. We conducted whole-genome sequencing analysis of 138 representative 3GC-R isolates previously collected from dairy farms in southwest England and confirmed by PCR to carry acquired 3GC-R genes. This analysis identified <i>bla</i> <sub>CTX-M</sub> (131 isolates encoding CTX-M-1, -14, -15, -and 32 and the novel variant CTX-M-214), <i>bla</i> <sub>CMY-2</sub> (6 isolates), and <i>bla</i> <sub>DHA-1</sub> (1 isolate). A highly conserved plasmid was identified in 73 isolates, representing 27 <i>E. coli</i> sequence types. This novel ∼220-kb IncHI2 plasmid carrying <i>bla</i> <sub>CTX-M-32</sub> was sequenced to closure and designated pMOO-32. It was found expe","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Dec","modification":"2025-04-20T00:15:19.509Z","creation":"2022-02-10T15:03:57.044Z"},"accession":"S-EPMC7755243","cross_references":{"pubmed":["33067197"],"doi":["10.1128/aem.01842-20","10.1128/AEM.01842-20"]}}