biostudies-literatureDudele ASteno Diabetes Center Aarhus (SDCA)Novo Nordisk FondenLundbeck Foundation579373https://www.ebi.ac.uk/biostudies/studies/S-EPMC7758475biostudies-literatureUnknown14Peripheral nerve function is metabolically demanding and nerve energy failure has been implicated in the onset and development of diabetic peripheral neuropathy and neuropathic pain conditions. Distal peripheral nerve oxygen supply relies on the distribution of red blood cells (RBCs) in just a few, nearby capillary-sized vessels and is therefore technically challenging to characterize. We developed an approach to characterize distal sural nerve hemodynamics in anesthetized, adult male mice using <i>in vivo</i> two-photon laser scanning microscopy. Our results show that RBC velocities in mouse sural nerve vessels are higher than those previously measured in mouse brain, and are sensitive to hindlimb temperatures. Nerve blood flow, measured as RBC flux, however, was similar to that of mouse Frontiers in neuroscienceSural Nerve Perfusion in Mice.PMC7758475304NNF14OC0011633R17-2007-1679R54-2010-5765Dudele AOstergaard LRasmussen PMfalseSural Nerve Perfusion in Mice.Peripheral nerve function is metabolically demanding and nerve energy failure has been implicated in the onset and development of diabetic peripheral neuropathy and neuropathic pain conditions. Distal peripheral nerve oxygen supply relies on the distribution of red blood cells (RBCs) in just a few, nearby capillary-sized vessels and is therefore technically challenging to characterize. We developed an approach to characterize distal sural nerve hemodynamics in anesthetized, adult male mice using <i>in vivo</i> two-photon laser scanning microscopy. Our results show that RBC velocities in mouse sural nerve vessels are higher than those previously measured in mouse brain, and are sensitive to hindlimb temperatures. Nerve blood flow, measured as RBC flux, however, was similar to that of mouse 2020-01-01T00:00:00Z20202025-04-05T12:54:51.789Z2025-04-05T12:54:51.789ZS-EPMC77584753336245410.3389/fnins.2020.579373