{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9(12)"],"submitter":["Hauck AK"],"pubmed_abstract":["Oxidative stress is a hallmark of metabolic disease, though the mechanisms that define this link are not fully understood. Irreversible modification of proteins by reactive lipid aldehydes (protein carbonylation) is a major consequence of oxidative stress in adipose tissue and the substrates and specificity of this modification are largely unexplored. Here we show that histones are avidly modified by 4-hydroxynonenal (4-HNE) in vitro and in vivo. Carbonylation of histones by 4-HNE increased with age in male flies and visceral fat depots of mice and was potentiated in genetic (ob/ob) and high-fat feeding models of obesity. Proteomic evaluation of in vitro 4-HNE- modified histones led to the identification of both Michael and Schiff base adducts. In contrast, mapping of sites in vivo from ob"],"journal":["Antioxidants (Basel, Switzerland)"],"pagination":["E1210"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7761391"],"repository":["biostudies-literature"],"pubmed_title":["Histone Carbonylation Is a Redox-Regulated Epigenomic Mark That Accumulates with Obesity and Aging."],"pmcid":["PMC7761391"],"pubmed_authors":["Bernlohr DA","Zhou T","Hauck AK","Chen Y","Upadhyay A","Sun Y","O'Connor MB"],"additional_accession":[]},"is_claimable":false,"name":"Histone Carbonylation Is a Redox-Regulated Epigenomic Mark That Accumulates with Obesity and Aging.","description":"Oxidative stress is a hallmark of metabolic disease, though the mechanisms that define this link are not fully understood. Irreversible modification of proteins by reactive lipid aldehydes (protein carbonylation) is a major consequence of oxidative stress in adipose tissue and the substrates and specificity of this modification are largely unexplored. Here we show that histones are avidly modified by 4-hydroxynonenal (4-HNE) in vitro and in vivo. Carbonylation of histones by 4-HNE increased with age in male flies and visceral fat depots of mice and was potentiated in genetic (ob/ob) and high-fat feeding models of obesity. Proteomic evaluation of in vitro 4-HNE- modified histones led to the identification of both Michael and Schiff base adducts. In contrast, mapping of sites in vivo from ob","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Dec","modification":"2025-04-18T17:42:51.903Z","creation":"2021-02-20T16:41:22Z"},"accession":"S-EPMC7761391","cross_references":{"pubmed":["33271806"],"doi":["10.3390/antiox9121210"]}}