{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Taborska P"],"funding":["Ministry of Health, Czech Republic","Charles University in Prague"],"pagination":["E3766"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7765077"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(12)"],"pubmed_abstract":["CD8+ T cells protect against tumors and intracellular pathogens. The inflammatory cytokines IL-2, IL-15, and IL-7 are necessary for their expansion. However, elevated serum levels of these cytokines are often associated with cancer, poorer prognosis of cancer patients, and exhaustion of antigen-expanded CD8+ T cells. The impact of acute conditioning of antigen-expanded CD8+ T cells with these cytokines is unknown. Here, we generated antigen-expanded CD8+ T cells using dendritic cells and PC-3 cells. The cells were acutely (18-24 h) conditioned with IL-2 and either the GSK3β inhibitor TWS119, the mTORC1 inhibitor rapamycin, or the mTORC1/2 inhibitor Torin1, then their immediate and post-re-expansion (distal) cytokine responses after antigen rechallenge were evaluated. We found that acute IL"],"journal":["Cancers"],"pubmed_title":["Acute Conditioning of Antigen-Expanded CD8+ T Cells via the GSK3β-mTORC Axis Differentially Dictates Their Immediate and Distal Responses after Antigen Rechallenge."],"pmcid":["PMC7765077"],"funding_grant_id":["364218","AZV 16-28135A","PRIMUS/MED/12"],"pubmed_authors":["Smrz D","Stakheev D","Bartunkova J","Strizova Z","Taborska P","Svobodova H"],"additional_accession":[]},"is_claimable":false,"name":"Acute Conditioning of Antigen-Expanded CD8+ T Cells via the GSK3β-mTORC Axis Differentially Dictates Their Immediate and Distal Responses after Antigen Rechallenge.","description":"CD8+ T cells protect against tumors and intracellular pathogens. The inflammatory cytokines IL-2, IL-15, and IL-7 are necessary for their expansion. However, elevated serum levels of these cytokines are often associated with cancer, poorer prognosis of cancer patients, and exhaustion of antigen-expanded CD8+ T cells. The impact of acute conditioning of antigen-expanded CD8+ T cells with these cytokines is unknown. Here, we generated antigen-expanded CD8+ T cells using dendritic cells and PC-3 cells. The cells were acutely (18-24 h) conditioned with IL-2 and either the GSK3β inhibitor TWS119, the mTORC1 inhibitor rapamycin, or the mTORC1/2 inhibitor Torin1, then their immediate and post-re-expansion (distal) cytokine responses after antigen rechallenge were evaluated. We found that acute IL","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Dec","modification":"2025-04-18T17:41:57.549Z","creation":"2021-02-20T16:47:11Z"},"accession":"S-EPMC7765077","cross_references":{"pubmed":["33327544"],"doi":["10.3390/cancers12123766"]}}