{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chiou YY"],"funding":["Ministry of Science and Technology, Taiwan","NIGMS NIH HHS"],"pagination":["616802"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7768009"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14"],"pubmed_abstract":["The transcription-translation feedback loop (TTFL) is the core mechanism of the circadian rhythm. In mammalian cells, CLOCK-BMAL1 proteins activate the downstream genes by binding on the E-box sequence of the clock-controlled genes. Among these gene products, CRY1, CRY2, PER1, PER2, NR1D1, and NR1D2 can regulate the CLOCK-BMAL1-mediated transcription to form the feedback loop. However, the detailed mechanism of the TTFL is unclear because of the complicated inter-regulation of these proteins. Here, we generated a cell line lacking CRY1, CRY2, PER1, PER2, NR1D1, and NR1D2 (Cry/Per/Nr1d_KO) to study TTFL. We compared the Dbp transcription after serum-shock and dexamethasone-shock between Cry/Per/Nr1d_KO cells and cells expressing endogenous CRY (Per/Nr1d_KO) or NR1D (Cry/Per_KO). Furthermore"],"journal":["Frontiers in neuroscience"],"pubmed_title":["A Sextuple Knockout Cell Line System to Study the Differential Roles of CRY, PER, and NR1D in the Transcription-Translation Feedback Loop of the Circadian Clock."],"pmcid":["PMC7768009"],"funding_grant_id":["107-2311-B-005-012-MY3","R35 GM118102","106-2311-B-005-014"],"pubmed_authors":["Chiou YY","Yang Y","Sancar A","Li TY"],"additional_accession":[]},"is_claimable":false,"name":"A Sextuple Knockout Cell Line System to Study the Differential Roles of CRY, PER, and NR1D in the Transcription-Translation Feedback Loop of the Circadian Clock.","description":"The transcription-translation feedback loop (TTFL) is the core mechanism of the circadian rhythm. In mammalian cells, CLOCK-BMAL1 proteins activate the downstream genes by binding on the E-box sequence of the clock-controlled genes. Among these gene products, CRY1, CRY2, PER1, PER2, NR1D1, and NR1D2 can regulate the CLOCK-BMAL1-mediated transcription to form the feedback loop. However, the detailed mechanism of the TTFL is unclear because of the complicated inter-regulation of these proteins. Here, we generated a cell line lacking CRY1, CRY2, PER1, PER2, NR1D1, and NR1D2 (Cry/Per/Nr1d_KO) to study TTFL. We compared the Dbp transcription after serum-shock and dexamethasone-shock between Cry/Per/Nr1d_KO cells and cells expressing endogenous CRY (Per/Nr1d_KO) or NR1D (Cry/Per_KO). Furthermore","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020","modification":"2026-04-07T23:22:50.912Z","creation":"2025-06-01T01:05:09.877Z"},"accession":"S-EPMC7768009","cross_references":{"pubmed":["33381013"],"doi":["10.3389/fnins.2020.616802"]}}