biostudies-literatureUnknown15Iimori Y<h4>Introduction</h4>In-body tissue architecture (iBTA) technology, based on cell-free tissue engineering, can produces collagenous tissues for implantation by subcutaneous embedding a designed mold. The aim of this study was to evaluate the biocompatibility of iBTA-induced "Biosheet®" collagenous sheets, as scaffold materials for bladder reconstruction.<h4>Methods</h4>Canine Biosheet® implants were prepared by embedding molds into subcutaneous pouches in beagles for 8 weeks. A part of canine bladder wall was excised (2 × 2 cm) and repaired by patching the same sized autologous Biosheet®. The Biosheet® implants were harvested 4 weeks (n = 1) and 12 weeks (n = 3) after the implantation and evaluated histologically.<h4>Results</h4>No disruption of the patched Biosheet® implants or urinary leRegenerative therapy274-280https://www.ebi.ac.uk/biostudies/studies/S-EPMC7770416biostudies-literatureUrinary bladder reconstruction using autologous collagenous connective tissue membrane "Biosheet®" induced by in-body tissue architecture: A pilot study.PMC7770416Nagatani KNakayama YIwai ROkamoto MIimori YNakata MMie KAkiyoshi HInoue YFunayama-Iwai MNishida HfalseUrinary bladder reconstruction using autologous collagenous connective tissue membrane "Biosheet®" induced by in-body tissue architecture: A pilot study.<h4>Introduction</h4>In-body tissue architecture (iBTA) technology, based on cell-free tissue engineering, can produces collagenous tissues for implantation by subcutaneous embedding a designed mold. The aim of this study was to evaluate the biocompatibility of iBTA-induced "Biosheet®" collagenous sheets, as scaffold materials for bladder reconstruction.<h4>Methods</h4>Canine Biosheet® implants were prepared by embedding molds into subcutaneous pouches in beagles for 8 weeks. A part of canine bladder wall was excised (2 × 2 cm) and repaired by patching the same sized autologous Biosheet®. The Biosheet® implants were harvested 4 weeks (n = 1) and 12 weeks (n = 3) after the implantation and evaluated histologically.<h4>Results</h4>No disruption of the patched Biosheet® implants or urinary le2020-01-01T00:00:00Z2020 Dec2025-04-19T08:13:33.276Z2021-02-20T20:51:17ZS-EPMC77704163342622910.1016/j.reth.2020.10.006