{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Huang X"],"funding":["National Institute of Allergy and Infectious Diseases","National Institute of Environmental Health Sciences","NIEHS NIH HHS","NIH Office of the Director","NIAID NIH HHS","Division of Biological Infrastructure","National Cancer Institute","NCI NIH HHS","Division of Information and Intelligent Systems","National Institute of General Medical Sciences","NIGMS NIH HHS","NIH HHS"],"pagination":["4844-4856"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7770890"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["19(12)"],"pubmed_abstract":["Despite considerable research progress on SARS-CoV-2, the direct zoonotic origin (intermediate host) of the virus remains ambiguous. The most definitive approach to identify the intermediate host would be the detection of SARS-CoV-2-like coronaviruses in wild animals. However, due to the high number of animal species, it is not feasible to screen all the species in the laboratory. Given that binding to ACE2 proteins is the first step for the coronaviruses to invade host cells, we propose a computational pipeline to identify potential intermediate hosts of SARS-CoV-2 by modeling the binding affinity between the Spike receptor-binding domain (RBD) and host ACE2. Using this pipeline, we systematically examined 285 ACE2 variants from mammals, birds, fish, reptiles, and amphibians, and found th"],"journal":["Journal of proteome research"],"pubmed_title":["Identifying the Zoonotic Origin of SARS-CoV-2 by Modeling the Binding Affinity between the Spike Receptor-Binding Domain and Host ACE2."],"pmcid":["PMC7770890"],"funding_grant_id":["R35 GM136422","P30ES017885","R01 AI134678","DBI2030790","IIS1901191","R01 GM083107","U24 CA210967","P30 ES017885","R01 GM116960","S10 OD026825","U24CA210967","R01 GM084222","AI134678","GM136422","S10OD026825"],"pubmed_authors":["Pearce R","Zhang Y","Huang X","Omenn GS","Zhang C"],"additional_accession":[]},"is_claimable":false,"name":"Identifying the Zoonotic Origin of SARS-CoV-2 by Modeling the Binding Affinity between the Spike Receptor-Binding Domain and Host ACE2.","description":"Despite considerable research progress on SARS-CoV-2, the direct zoonotic origin (intermediate host) of the virus remains ambiguous. The most definitive approach to identify the intermediate host would be the detection of SARS-CoV-2-like coronaviruses in wild animals. However, due to the high number of animal species, it is not feasible to screen all the species in the laboratory. Given that binding to ACE2 proteins is the first step for the coronaviruses to invade host cells, we propose a computational pipeline to identify potential intermediate hosts of SARS-CoV-2 by modeling the binding affinity between the Spike receptor-binding domain (RBD) and host ACE2. Using this pipeline, we systematically examined 285 ACE2 variants from mammals, birds, fish, reptiles, and amphibians, and found th","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Dec","modification":"2026-05-02T21:38:59.237Z","creation":"2026-04-07T18:30:52.403Z"},"accession":"S-EPMC7770890","cross_references":{"pubmed":["33175551"],"doi":["10.1021/acs.jproteome.0c00717"]}}