{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["15(12)"],"submitter":["Theart RP"],"pubmed_abstract":["Mitochondrial fission and fusion play an important role not only in maintaining mitochondrial homeostasis but also in preserving overall cellular viability. However, quantitative analysis based on the three-dimensional localisation of these highly dynamic mitochondrial events in the cellular context has not yet been accomplished. Moreover, it remains largely uncertain where in the mitochondrial network depolarisation is most likely to occur. We present the mitochondrial event localiser (MEL), a method that allows high-throughput, automated and deterministic localisation and quantification of mitochondrial fission, fusion and depolarisation events in large three-dimensional microscopy time-lapse sequences. In addition, MEL calculates the number of mitochondrial structures as well as their c"],"journal":["PloS one"],"pagination":["e0229634"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7773280"],"repository":["biostudies-literature"],"pubmed_title":["Mitochondrial event localiser (MEL) to quantitativelydescribe fission, fusion and depolarisation in the three-dimensional space."],"pmcid":["PMC7773280"],"pubmed_authors":["Kriel J","du Toit A","Theart RP","Loos B","Niesler TR"],"additional_accession":[]},"is_claimable":false,"name":"Mitochondrial event localiser (MEL) to quantitativelydescribe fission, fusion and depolarisation in the three-dimensional space.","description":"Mitochondrial fission and fusion play an important role not only in maintaining mitochondrial homeostasis but also in preserving overall cellular viability. However, quantitative analysis based on the three-dimensional localisation of these highly dynamic mitochondrial events in the cellular context has not yet been accomplished. Moreover, it remains largely uncertain where in the mitochondrial network depolarisation is most likely to occur. We present the mitochondrial event localiser (MEL), a method that allows high-throughput, automated and deterministic localisation and quantification of mitochondrial fission, fusion and depolarisation events in large three-dimensional microscopy time-lapse sequences. In addition, MEL calculates the number of mitochondrial structures as well as their c","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020","modification":"2025-04-22T09:35:22.13Z","creation":"2021-02-20T20:50:22Z"},"accession":"S-EPMC7773280","cross_references":{"pubmed":["33378337"],"doi":["10.1371/journal.pone.0229634"]}}