biostudies-literatureKolmert JMedical Research CouncilNational Institute for Health Research (NIHR)37-53https://www.ebi.ac.uk/biostudies/studies/S-EPMC7781128biostudies-literatureUnknown203(1)<b>Rationale:</b> New approaches are needed to guide personalized treatment of asthma.<b>Objectives:</b> To test if urinary eicosanoid metabolites can direct asthma phenotyping.<b>Methods:</b> Urinary metabolites of prostaglandins (PGs), cysteinyl leukotrienes (CysLTs), and isoprostanes were quantified in the U-BIOPRED (Unbiased Biomarkers for the Prediction of Respiratory Diseases Outcomes) study including 86 adults with mild-to-moderate asthma (MMA), 411 with severe asthma (SA), and 100 healthy control participants. Validation was performed internally in 302 participants with SA followed up after 12-18 months and externally in 95 adolescents with asthma.<b>Measurement and Main Results:</b> Metabolite concentrations in healthy control participants were unrelated to age, body mass index, aAmerican journal of respiratory and critical care medicineUrinary Leukotriene E&lt;sub&gt;4&lt;/sub&gt; and Prostaglandin D&lt;sub&gt;2&lt;/sub&gt; Metabolites Increase in Adult and Childhood Severe Asthma Characterized by Type 2 Inflammation. A Clinical Observational Study.PMC7781128NIHR200199MR/N005953/1Hedlin GD'Amico ABoedigheimer MJGuo YJames AGomez CCunoosamy DPratico GRowe ASkipp PJCaruso MAhmed HPavlidis SPostle ABonnelykke KAdcock IMBrinkman PErzen DPahus LChanez PBucchioni ESandstrom TMeiser ASchofield JPRFleming LJLefaudeux DDjukanovic RMasefield SMatthews JGFrey Uvan Geest MMiddelveld RJMSinger FKrug NBood JBigler JWheelock AMAlving KBurg DMurray CSOstling JBaribaud FVissing NHMores NHashimoto SPowel PHigenbottam TWagers SSThornton BKnox AJFichtner KThorngren JOvan Aalderen WMBisgaard HHowarth PBush ABehndig ASmicker MWeiszhart ZRoberts AFitch NKolmert JEricsson MDahlen SEDe Meulder BMikus MBrandsma JFormaggio ERoberts GFowler SJHolweg CTJManta AMazein AGahlemann MMiralpeix MSeibold WWheelock CEMusial JWilson SJSousa ARSterk PJLutter RRao NSun KBates SPuigValls MMyles DCorfield JGeiser TAuffray CMontuschi PKnowles RGNordlund BDahlen BChung KFKonradsen JRBansal ATSigmund RLoza MJPandis IHaughney JBel EHBilling BWagener AHErpenbeck VJHorvath IBalgoma DGoss VHekking PWLazarinis NHohlfeld JMSjodin MSelby ABakke PSChaiboonchoe AUddin MVestbo JSanak MRiley JHU-BIOPRED Study Group, on behalf of the U-BIOPRED Study GroupShaw DECompton CHfalseUrinary Leukotriene E&lt;sub&gt;4&lt;/sub&gt; and Prostaglandin D&lt;sub&gt;2&lt;/sub&gt; Metabolites Increase in Adult and Childhood Severe Asthma Characterized by Type 2 Inflammation. A Clinical Observational Study.<b>Rationale:</b> New approaches are needed to guide personalized treatment of asthma.<b>Objectives:</b> To test if urinary eicosanoid metabolites can direct asthma phenotyping.<b>Methods:</b> Urinary metabolites of prostaglandins (PGs), cysteinyl leukotrienes (CysLTs), and isoprostanes were quantified in the U-BIOPRED (Unbiased Biomarkers for the Prediction of Respiratory Diseases Outcomes) study including 86 adults with mild-to-moderate asthma (MMA), 411 with severe asthma (SA), and 100 healthy control participants. Validation was performed internally in 302 participants with SA followed up after 12-18 months and externally in 95 adolescents with asthma.<b>Measurement and Main Results:</b> Metabolite concentrations in healthy control participants were unrelated to age, body mass index, a2021-01-01T00:00:00Z2021 Jan2026-06-02T16:19:45.32Z2022-02-09T17:36:40.447ZS-EPMC77811283266726110.1164/rccm.201909-1869OC