{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Xuan J"],"funding":["National Natural Science Foundation of China","Jiangsu Province 333 High-level Talent Training Project","Medical Research Project of Jiangsu Commission of Health"],"pagination":["3480-3490"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC7781632"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["19(24)"],"pubmed_abstract":["Crocin is the main monomer of saffron, which is a momentous component of traditional Chinese medicine Lang Qing A Ta. Here, we tried to probe into the role of crocin in liver fibrosis. Hematoxylin-eosin staining and Sirius Red staining were used to observe the pathological changes of liver tissues. After hepatic stellate cells (HSCs) were isolated from liver tissues, lnc-LFAR1, MTF-1, GDNF, and α-SMA expressions were detected by qRT-PCR and western blot. Immunohistochemistry and immunofluorescence were used to detect α-SMA expression. Chromatin immunoprecipitation was used to analyze the binding of MTF-1 to the GDNF promoter. Moreover, the dual-luciferase reporter gene, RNA pull-down, and RNA immunoprecipitation were used to clarify the interaction between MTF-1 and GDNF, lnc-LFAR1 and MTF"],"journal":["Cell cycle (Georgetown, Tex.)"],"pubmed_title":["Crocin inhibits the activation of mouse hepatic stellate cells via the lnc-LFAR1/MTF-1/GDNF pathway."],"pmcid":["PMC7781632"],"funding_grant_id":["BRA2018408","No. 81704196","H2019073"],"pubmed_authors":["Qiu Y","Yang Y","Shao M","Wang F","Qin F","Xuan J","Zhai Q","Zhu D","Cheng Z"],"additional_accession":[]},"is_claimable":false,"name":"Crocin inhibits the activation of mouse hepatic stellate cells via the lnc-LFAR1/MTF-1/GDNF pathway.","description":"Crocin is the main monomer of saffron, which is a momentous component of traditional Chinese medicine Lang Qing A Ta. Here, we tried to probe into the role of crocin in liver fibrosis. Hematoxylin-eosin staining and Sirius Red staining were used to observe the pathological changes of liver tissues. After hepatic stellate cells (HSCs) were isolated from liver tissues, lnc-LFAR1, MTF-1, GDNF, and α-SMA expressions were detected by qRT-PCR and western blot. Immunohistochemistry and immunofluorescence were used to detect α-SMA expression. Chromatin immunoprecipitation was used to analyze the binding of MTF-1 to the GDNF promoter. Moreover, the dual-luciferase reporter gene, RNA pull-down, and RNA immunoprecipitation were used to clarify the interaction between MTF-1 and GDNF, lnc-LFAR1 and MTF","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Dec","modification":"2025-04-20T03:42:28.412Z","creation":"2022-02-11T13:27:46.519Z"},"accession":"S-EPMC7781632","cross_references":{"pubmed":["33295246"],"doi":["10.1080/15384101.2020.1848064"]}}